Biological and biochemical characterization of a cloned Leu- 3- cell surviving infection with the acquired immune deficiency syndrome retrovirus

T. M. Folks, D. Powell, M. Lightfoote, S. Koenig, A. S. Fauci, S. Benn, A. Rabson, D. Daugherty, H. E. Gendelman, M. D. Hoggan

Research output: Contribution to journalArticle

321 Citations (Scopus)

Abstract

Leu-3- cells that survive infection with the acquired immune deficiency syndrome (AIDS) retrovirus can be induced with IUdR to express infectious virus (1). A cellular clone (8E5), isolated by limiting dilution of a mass culture of survivor cells, was found to contain a single, integrated provirus that was constitutively expressed. Although IUdR treatment of 8E5 cells failed to induce infectious virus, cocultivation with Leu-3+ cells generated the characteristic syncytia associated with acute AIDS retrovirus infection. The single integrated copy of proviral DNA directs the synthesis of all major viral structural proteins except p64, as monitored by immunoblotting. The relationship of the 8E5 clone to viral latency and persistence is discussed.

Original languageEnglish (US)
Pages (from-to)280-290
Number of pages11
JournalJournal of Experimental Medicine
Volume164
Issue number1
StatePublished - 1986
Externally publishedYes

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Retroviridae
Idoxuridine
Acquired Immunodeficiency Syndrome
Clone Cells
Infection
Retroviridae Infections
Virus Latency
Viruses
Viral Structural Proteins
Proviruses
Giant Cells
Coculture Techniques
Immunoblotting
Cell Culture Techniques
DNA

ASJC Scopus subject areas

  • Immunology

Cite this

Folks, T. M., Powell, D., Lightfoote, M., Koenig, S., Fauci, A. S., Benn, S., ... Hoggan, M. D. (1986). Biological and biochemical characterization of a cloned Leu- 3- cell surviving infection with the acquired immune deficiency syndrome retrovirus. Journal of Experimental Medicine, 164(1), 280-290.

Biological and biochemical characterization of a cloned Leu- 3- cell surviving infection with the acquired immune deficiency syndrome retrovirus. / Folks, T. M.; Powell, D.; Lightfoote, M.; Koenig, S.; Fauci, A. S.; Benn, S.; Rabson, A.; Daugherty, D.; Gendelman, H. E.; Hoggan, M. D.

In: Journal of Experimental Medicine, Vol. 164, No. 1, 1986, p. 280-290.

Research output: Contribution to journalArticle

Folks, TM, Powell, D, Lightfoote, M, Koenig, S, Fauci, AS, Benn, S, Rabson, A, Daugherty, D, Gendelman, HE & Hoggan, MD 1986, 'Biological and biochemical characterization of a cloned Leu- 3- cell surviving infection with the acquired immune deficiency syndrome retrovirus', Journal of Experimental Medicine, vol. 164, no. 1, pp. 280-290.
Folks, T. M. ; Powell, D. ; Lightfoote, M. ; Koenig, S. ; Fauci, A. S. ; Benn, S. ; Rabson, A. ; Daugherty, D. ; Gendelman, H. E. ; Hoggan, M. D. / Biological and biochemical characterization of a cloned Leu- 3- cell surviving infection with the acquired immune deficiency syndrome retrovirus. In: Journal of Experimental Medicine. 1986 ; Vol. 164, No. 1. pp. 280-290.
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