Biogenesis of Marek's disease (Type II leukosis) virus in vitro: electron microscopy and immunological study

F. Hamdy, M. Sevoian, S. C. Holt

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

The kinetic events involved in Marek's disease herpesvirus infection of avian cell culture were investigated by assaying viral infectivity and antigenicity as well as by electron microscopy during the infectious cycle. The levels of viral infectivity and complement fixing (CF) antigens revealed that the rates of appearance of infectious particles and CF antigens were not synchronous. Viral specific CF antigen could be detected 5 hr after infection, whereas viral infectivity or the appearance of viral particles could be demonstrated only after 10 hr of infection. High proportions of the recovered CF antigens during the various stages of the infectious cycle were found to be soluble and did not sediment with the virus particles. Cytological analysis of the developmental stages of the JM virus infected cells by thin sectioning and electron microscopy revealed that at 8 hr small particles approximately 35 nm in diameter appeared in the cell nuclei. The appearance of nucleocapsids occurred at 10 hr, and these were of varying shapes; however, all were approximately 100 nm in diameter. At approximately 18 hr postinfection, mature virus particles were observed. Viral maturation of the immature particles occurred by the acquisition of envelope from the inner leaflet of the nuclear membrane or from the cytoplasmic membrane of the cell. (33 references.)

Original languageEnglish (US)
Pages (from-to)740-749
Number of pages10
JournalInfection and immunity
Volume9
Issue number4
StatePublished - Dec 1 1974
Externally publishedYes

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

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