Biodistribution and imaging studies of technetium-99m-labeled liposomes in rats with focal infection

B. Goins, R. Klipper, A. S. Rudolph, R. O. Cliff, R. Blumhardt, W. T. Phillips

Research output: Contribution to journalArticlepeer-review

65 Scopus citations

Abstract

We have recently developed a procedure to label liposomes containing reduced glutathione (GSH) with 99mTc using the lipophilic chelator, hexamethylpropyleneamine oxime (HMPAO). In the present study, we evaluated the use of 99mTc-liposomes to detect focal infection sites in rats. Rats were infected in the thigh by intramuscular injection with Staphylococcus aureus followed 24 hr later by an intravenous injection of 99mTc- liposomes, 67Ga-citrate, or 99mTc-human serum albumin (HSA). The animals were imaged under a gamma camera and subsequently killed at 4, 24 or 48 hr for tissue biodistribution studies. In contrast to infected rats receiving 67Ga-citrate or 99mTc-HSA, abscesses were prominently localized within 2 hr in rats after 99mTc-liposome injection, and continued to increase in activity up to 24 hr. Abscess-to-muscle ratios calculated from 24-hr biodistribution data obtained from tissue sampling were 35.3 ± 7.6 for 99mTc-liposomes, 4.1 ± 0.7 for 67Ga-citrate and 8.0 ± 1.0 for 99mTc-HSA. These studies show the potential of using 99mTc-liposomes to localize infection.

Original languageEnglish (US)
Pages (from-to)2160-2168
Number of pages9
JournalJournal of Nuclear Medicine
Volume34
Issue number12
StatePublished - Jan 1 1993

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

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