TY - JOUR
T1 - Biochemical mechanism of DSB end resection and its regulation
AU - Daley, James M.
AU - Niu, Hengyao
AU - Miller, Adam S.
AU - Sung, Patrick
N1 - Funding Information:
The studies in our laboratory have been supported by research grants and a program project grant (SBDR) from the National Institutes of Health. We apologize to colleagues whose work is not cited owing to space limitation.
Publisher Copyright:
© 2015 Elsevier B.V.
PY - 2015/8/1
Y1 - 2015/8/1
N2 - DNA double-strand breaks (DSBs) in cells can undergo nucleolytic degradation to generate long 3' single-stranded DNA tails. This process is termed DNA end resection, and its occurrence effectively commits to break repair via homologous recombination, which entails the acquisition of genetic information from an intact, homologous donor DNA sequence. Recent advances, prompted by the identification of the nucleases that catalyze resection, have revealed intricate layers of functional redundancy, interconnectedness, and regulation. Here, we review the current state of the field with an emphasis on the major questions that remain to be answered. Topics addressed will include how resection initiates via the introduction of an endonucleolytic incision close to the break end, the molecular mechanism of the conserved MRE11 complex in conjunction with Sae2/CtIP within such a model, the role of BRCA1 and 53BP1 in regulating resection initiation in mammalian cells, the influence of chromatin in the resection process, and potential roles of novel factors.
AB - DNA double-strand breaks (DSBs) in cells can undergo nucleolytic degradation to generate long 3' single-stranded DNA tails. This process is termed DNA end resection, and its occurrence effectively commits to break repair via homologous recombination, which entails the acquisition of genetic information from an intact, homologous donor DNA sequence. Recent advances, prompted by the identification of the nucleases that catalyze resection, have revealed intricate layers of functional redundancy, interconnectedness, and regulation. Here, we review the current state of the field with an emphasis on the major questions that remain to be answered. Topics addressed will include how resection initiates via the introduction of an endonucleolytic incision close to the break end, the molecular mechanism of the conserved MRE11 complex in conjunction with Sae2/CtIP within such a model, the role of BRCA1 and 53BP1 in regulating resection initiation in mammalian cells, the influence of chromatin in the resection process, and potential roles of novel factors.
KW - Double-strand breaks
KW - Helicases
KW - Nucleases
KW - Recombination
KW - Resection
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U2 - 10.1016/j.dnarep.2015.04.015
DO - 10.1016/j.dnarep.2015.04.015
M3 - Article
C2 - 25956866
AN - SCOPUS:84938483671
SN - 1568-7864
VL - 32
SP - 66
EP - 74
JO - DNA Repair
JF - DNA Repair
ER -