Biochemical characterization of cholesterol-reducing Eubacterium

G. E. Mott, A. W. Brinkley, C. L. Mersinger

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

The authors characterized 2 isolates of cholesterol-reducing Eubacterium by conducting conventional biochemical tests and by testing various sterols and glycerolipids as potential growth factors. In media containing cholesterol and plasmenylethanolamine, the tests for nitrate reduction, indole production, and gelatin and starch hydrolyses were negative, and no acid was produced from any of 22 carbohydrates. Both isolates hydrolyzed esculin to esculetin, indicating β-glycosidase activity. In addition to plasmenylethanolamine, 5 other lipids which contain an alkenyl ether residue supported growth of Eubacterium strain 403 in a lecithin-cholesterol base medium. Of 6 steroids tested, cholesterol, cholest-4-en-3-one, cholest-4-en-3β-ol (allocholesterol), and androst-5-en-3β-ol-17-one supported growth of Eubacterium strain 403. All 4 steroids were reduced to the 3β-ol, 5β-H products. The δ5 steroids cholest-5-en-3α-ol (epicholesterol) and 22, 23-bisnor-5-cholenic acid-3β-ol were not reduced and did not support growth of the Eubacterium strain.

Original languageEnglish (US)
Pages (from-to)1017-1022
Number of pages6
JournalApplied and Environmental Microbiology
Volume40
Issue number6
DOIs
StatePublished - 1980

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

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