Biochemical and molecular basis of differentiation in plant tissue culture

Basic understanding of plant cell proliferation and differentiation is imminent for applying modern techniques of genetic transformations. Although remarkable progress has been made in the area of gene transfer technology, little is known as to how plant cells differentiate in cultures. Plant tissue culture has therefore remained an empirical science. In this review, an attempt has been made to assimilate the current knowledge of the various biochemical and molecular parameters, which play an important role in differentiation.