Bioautography: A general method for the visualization of isozymes

Susan L. Naylor, Robert J. Klebe

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

A new method has been developed for visualization of isozymes which are difficult or impossible to detect with standard histochemical or autoradiographic methods. The principle of this method, bioautography, is the use of a microbial reagent to locate an enzyme after gel electrophoresis. When bioautography was compared to other staining procedures, the bioautographic method yielded identical results to those observed by the histochemical method for lactate dehydrogenase (LDH) or by the autoradiographic method for the adenine phosphoribosyl-transferase (APRT). Using the bioautographic method, stains for enzymes which could not be visualized by any other procedure have been developed: argininosuccinate lyase and branched-chain aminotransferase. By employing appropriately genetically marked bacterial strains, it should be possible to develop new isozyme stains for a large number of unstudied isozymes.

Original languageEnglish (US)
Pages (from-to)1193-1211
Number of pages19
JournalBiochemical Genetics
Volume15
Issue number11-12
DOIs
StatePublished - Dec 1977
Externally publishedYes

Keywords

  • biological assay
  • electrophoresis
  • histochemistry
  • isozymes
  • somatic cell genetics

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Biochemistry
  • Molecular Biology
  • Genetics

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