Bioautographic visualization of dihydrofolate reductase in enzyme overproducing BHK mutants

S. L. Naylor, J. K. Townsend, R. J. Klebe

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


A bioautographic procedure has been developed for the visualization of the isozymes of dihydrofolate reductase (DHFR, E.C. In addition to detecting electrophoretically separated enzymes, bioautography was utilized to visualize DHFR after isoelectric focusing on polyacrylamide gels. Both zone electrophoresis and isoelectric focusing were used to compare wildtype BHK cells to mutants which overproduce dihydrofolate reductase. In agreement with other physical data, the BHK-A5 overproduction mutant appears to produce more dihydrofolate reductase of the same electrophoretic mobility and isoelectric point as wild type cells.

Original languageEnglish (US)
Pages (from-to)199-206
Number of pages8
JournalBiochemical Genetics
Issue number1-2
StatePublished - Feb 1980
Externally publishedYes


  • bioautography
  • dihydrofolate reductase
  • electrophoresis
  • mutant cells

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Biochemistry
  • Molecular Biology
  • Genetics


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