Bioactive nitric oxide concentration does not increase during reactive hyperemia in human skin

J. L. Zhao, P. E. Pergola, L. J. Roman, Dean L Kellogg Jr

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

This study examined whether nitric oxide (NO) is involved in the cutaneous response to reactive hyperemia (RH) in the human forearm. We enrolled seven healthy volunteers. NO concentrations were monitored using a NO selective amperometric electrode (ISO-NOP200, World Precision Instruments) inserted into the skin of the forearm. Laser-Doppler flowmetry (Moor Instruments) was used for monitoring skin blood flow (SkBF) at the same site. SkBF and NO levels were monitored and recorded continuously throughout the experiment. An intradermal microdialysis probe was inserted adjacent to the NO electrode for drug delivery. Data collection began 140 min after the NO electrodes and microdialysis probes were inserted. RH was achieved by the inflation of a blood pressure cuff to 25 mmHg above systolic pressure for 7 min after which the pressure in the cuff was abruptly released. Acetylcholine (ACh) was given by microdialysis probe at the end of RH study to verify the ability of the electrode system to detect changes in the NO concentration. SkBF and NO data before RH and immediately, 2, 5, 7, and 10 min after cuff deflation were used for analysis. SkBF increased immediately after release of the occlusion (P < 0.0001) and remained elevated for 2 min. No significant NO changes occurred with the increases in LDF. ACh induced increases in both SkBF and NO (P < 0.000 and P < 0.037, respectively). We conclude that RH increases SkBF by mechanisms that do not require a measurable increase in NO concentrations.

Original languageEnglish (US)
Pages (from-to)628-632
Number of pages5
JournalJournal of Applied Physiology
Volume96
Issue number2
DOIs
StatePublished - Feb 2004

Fingerprint

Hyperemia
Nitric Oxide
Skin
Microdialysis
Electrodes
Forearm
Acetylcholine
Blood Pressure
Laser-Doppler Flowmetry
Economic Inflation
Healthy Volunteers
Pressure

Keywords

  • Amperometric electrode
  • Laser-Doppler flowmetry
  • Skin blood flow
  • Vasodilation

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Bioactive nitric oxide concentration does not increase during reactive hyperemia in human skin. / Zhao, J. L.; Pergola, P. E.; Roman, L. J.; Kellogg Jr, Dean L.

In: Journal of Applied Physiology, Vol. 96, No. 2, 02.2004, p. 628-632.

Research output: Contribution to journalArticle

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