Binding of rat ribosomal proteins to yeast 5.8S ribosomal ribonucleic acid

John C. Lee, Beth Henry

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

5.8 S RNA-protein complexes were prepared using purified yeast 5.8 S RNA and proteins from the large ribosomal subunit of rat liver. Formation of such hybrid complexes, as measured by Miffipore filtration, was dependent on protein concentration. Binding of proteins to the RNA could approach saturation. Such complexes were isolated from sucrose density gradient centrifugation and shown to contain proteins L6, L8, L19, L35 and L35a. These proteins were identified by their molecular weights on polyacrylamide gels containing dodecylsulfate and their mobilities on two dimensional polyacrylamide gels.

Original languageEnglish (US)
Pages (from-to)2199-2207
Number of pages9
JournalNucleic Acids Research
Volume10
Issue number7
DOIs
StatePublished - Apr 10 1982

Fingerprint

Ribosomal Proteins
RNA
Yeast
Rats
Yeasts
Protein S
Proteins
Protein
L 35
Large Ribosome Subunits
RNA-Binding Proteins
Density Gradient Centrifugation
Polyacrylates
Sucrose
Gels
Molecular Weight
Centrifugation
Liver
Sugar (sucrose)
Carrier Proteins

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

Cite this

Binding of rat ribosomal proteins to yeast 5.8S ribosomal ribonucleic acid. / Lee, John C.; Henry, Beth.

In: Nucleic Acids Research, Vol. 10, No. 7, 10.04.1982, p. 2199-2207.

Research output: Contribution to journalArticle

Lee, John C. ; Henry, Beth. / Binding of rat ribosomal proteins to yeast 5.8S ribosomal ribonucleic acid. In: Nucleic Acids Research. 1982 ; Vol. 10, No. 7. pp. 2199-2207.
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