TY - JOUR
T1 - Binding of rat ribosomal proteins to yeast 5.8S ribosomal ribonucleic acid
AU - Lee, John C.
AU - Henry, Beth
N1 - Funding Information:
This research was supported by the Robert A. Welch Foundation (AQ-541) and NIH.
PY - 1982/4/10
Y1 - 1982/4/10
N2 - 5.8 S RNA-protein complexes were prepared using purified yeast 5.8 S RNA and proteins from the large ribosomal subunit of rat liver. Formation of such hybrid complexes, as measured by Miffipore filtration, was dependent on protein concentration. Binding of proteins to the RNA could approach saturation. Such complexes were isolated from sucrose density gradient centrifugation and shown to contain proteins L6, L8, L19, L35 and L35a. These proteins were identified by their molecular weights on polyacrylamide gels containing dodecylsulfate and their mobilities on two dimensional polyacrylamide gels.
AB - 5.8 S RNA-protein complexes were prepared using purified yeast 5.8 S RNA and proteins from the large ribosomal subunit of rat liver. Formation of such hybrid complexes, as measured by Miffipore filtration, was dependent on protein concentration. Binding of proteins to the RNA could approach saturation. Such complexes were isolated from sucrose density gradient centrifugation and shown to contain proteins L6, L8, L19, L35 and L35a. These proteins were identified by their molecular weights on polyacrylamide gels containing dodecylsulfate and their mobilities on two dimensional polyacrylamide gels.
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U2 - 10.1093/nar/10.7.2199
DO - 10.1093/nar/10.7.2199
M3 - Article
C2 - 7045808
AN - SCOPUS:0020478921
VL - 10
SP - 2199
EP - 2207
JO - Nucleic Acids Research
JF - Nucleic Acids Research
SN - 0305-1048
IS - 7
ER -