Binding of benzo(a)pyrene derivatives to specific proteins in nuclei of intact hamster embryo Cells

Michael C. MacLeod, Betty K. Mansfield, Thomas J. Slaga, K. Selkirk James, Arend Kootstra

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Abstract

In hamster embryo cells incubated for 24 hr with 4 µ5m [3H]benzo(a)pyrene, a major portion of the nonextractable radioactivity in nuclear preparations copurifies with the protein fraction. When these proteins are analyzed by sodium dodecyl sulfate:polyacrylamide gel electrophoresis, significant variations in the labeling intensities of the various proteins are seen. Control experiments demonstrate that the labeling is due to covalent binding to protein. Histones H3 and H2A are heavily labeled while the other histones of the nucleosome core, H2B and H4, are devoid of radioactivity. Large amounts of label are associated with proteins with mobilities similar to the very lysine-rich histones H1. However, the results of differential extraction experiments suggest that the labeled proteins do not belong to either the H1 or the high-mobility-group class of chromosomal proteins. During 6 hr of inhibition of protein synthesis by cycloheximide, the metabolism of [3H]-benzo(a)pyrene, as monitored by high-pressure liquid chromatography, remained normal. Patterns of labeling of nuclear proteins after 3 or 6 hr were identical in the presence and absence of cycloheximide. This finding strongly suggests that binding of benzo(a)pyrene derivatives to nuclear proteins occurs in situ.

Original languageEnglish (US)
Pages (from-to)4080-4086
Number of pages7
JournalCancer Research
Volume41
Issue number10
Publication statusPublished - Oct 1 1981

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ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

MacLeod, M. C., Mansfield, B. K., Slaga, T. J., James, K. S., & Kootstra, A. (1981). Binding of benzo(a)pyrene derivatives to specific proteins in nuclei of intact hamster embryo Cells. Cancer Research, 41(10), 4080-4086.