Benzo(a)pyrene Metabolism in Primary Cultures of Mouse Epidermal Cells and Untransformed and Transformed Epidermal Cell Lines

John DiGiovanni, Jody M. Singer, Don R. Miller, Aurora Viaje, Thomas J. Slaga

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

The metabolism of [3H]benzo(a)pyrene [B(a)P] by cultures of primary mouse epidermal cells and untransformed and transformed epidermal cell lines was investigated. All three cell types effectively metabolized [3H]B(a)P. The major organic solvent-extractable metabolites found intracellularly in primary cultures were frans-7,8-dihydro-7,8-dihydroxybenzo(a)-pyrene and 3-hydroxybenzo(a)pyrene, although quantities of 9-hydroxybenzo(a)pyrene, frans-9,10-dihydro-9,10-dihydroxybenzo(a)pyrene, and quinones also were present. The major organic solvent-soluble metabolites found in the extracellular medium were trans- 9,10-dihydro-9,10-dihydroxy-benzo(a)pyrene and frans-7,8-dihydro-7,8-dihydroxybenzo(a)-pyrene, with smaller quantities of unconjugated phenols and quinones. The major water-soluble metabolites found in the extracellular medium were conjugated with glucuronic acid [primarily 3-hydroxybenzo(a)pyrene and several quinones]. No sulfate conjugates of [3H]B(a)P metabolites were detected. [3H]B(a)P metabolism was similar in cultures of untransformed and transformed epidermal cell lines but differed from the primary cultures. The major intracellular and extracellular organic solvent-soluble metabolites were diols. Little or no unconjugated phenols were detected. Both the untransformed and transformed epidermal cell lines converted [3H]B(a)P to water-soluble metabolites, primarily glucuronide conjugates. In contrast to the primary cells, a major pathway of frans-7,8-dihydro-7,8-dihydroxybenzo(a)pyrene metabolism in the untransformed and transformed cell lines was to a glucuronide conjugate. Primary mouse epidermal cells provide an important model system for studying factors affecting the activation and detoxification of hydrocarbon carcinogens.

Original languageEnglish (US)
Pages (from-to)2579-2586
Number of pages8
JournalCancer Research
Volume42
Issue number7
StatePublished - Jul 1 1982
Externally publishedYes

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Fingerprint Dive into the research topics of 'Benzo(a)pyrene Metabolism in Primary Cultures of Mouse Epidermal Cells and Untransformed and Transformed Epidermal Cell Lines'. Together they form a unique fingerprint.

Cite this