TY - JOUR
T1 - BCL-6 mutations in pulmonary lymphoproliferative disorders
T2 - Demonstration of an aberrant immunological reaction in HIV-related lymphoid interstitial pneumonia
AU - Kurosu, Katsushi
AU - Weiden, Michael D.
AU - Takiguchi, Yuichi
AU - Rom, William N.
AU - Yumoto, Norio
AU - Jaishree, Jagirdar
AU - Nakata, Koh
AU - Kasahara, Yasunori
AU - Tanabe, Nobuhiro
AU - Tatsumi, Koichiro
AU - Mikata, Atsuo
AU - Kuriyama, Takayuki
PY - 2004/6/1
Y1 - 2004/6/1
N2 - We used a PCR and sequence procedure to analyze the Ig VH gene and the mutations in the 5′ regulatory regions of BCL-6 genes in pulmonary lymphoproliferative disorders (mucosa-associated lymphoid tissue (MALT) lymphoma, HIV-related, EBV-related, and virus-negative lymphocytic interstitial pneumonia (LIP)). Eight of 20 (40%) pulmonary MALT lymphoma and 10 of 20 LIP (5 of 5 (100%) HIV-related, 2 of 5 (40%) EBV-related, and 3 of 10 (30%) virus-negative LIP) cases showed BCL-6 gene mutations. Intraclonal heterogeneity of the BCL-6 mutations was observed only in pulmonary MALT lymphoma cases whose Ig VH genes also showed intraclonal heterogeneity. Ongoing BCL-6 mutations might reflect re-entry into a germinal center pathway to further mutations. BCL-6 mutations in pulmonary MALT lymphoma and HIV-negative LIP showed some features (high transition to transversion ratio, standard polarity, and RGYW/WRCY bias) of Ig VH gene hypermutation, leading to the view that pulmonary MALT lymphomas and HIV-negative LIP are under the influence of germinal center hypermutation mechanisms. Because BCL-6 mutations in HIV-related LIP cases did not demonstrate features of Ig VH gene hypermutation, immunological reactions in HIV-related LIP are the result of a process different from that found in HIV-negative pulmonary lymphoproliferative disorders.
AB - We used a PCR and sequence procedure to analyze the Ig VH gene and the mutations in the 5′ regulatory regions of BCL-6 genes in pulmonary lymphoproliferative disorders (mucosa-associated lymphoid tissue (MALT) lymphoma, HIV-related, EBV-related, and virus-negative lymphocytic interstitial pneumonia (LIP)). Eight of 20 (40%) pulmonary MALT lymphoma and 10 of 20 LIP (5 of 5 (100%) HIV-related, 2 of 5 (40%) EBV-related, and 3 of 10 (30%) virus-negative LIP) cases showed BCL-6 gene mutations. Intraclonal heterogeneity of the BCL-6 mutations was observed only in pulmonary MALT lymphoma cases whose Ig VH genes also showed intraclonal heterogeneity. Ongoing BCL-6 mutations might reflect re-entry into a germinal center pathway to further mutations. BCL-6 mutations in pulmonary MALT lymphoma and HIV-negative LIP showed some features (high transition to transversion ratio, standard polarity, and RGYW/WRCY bias) of Ig VH gene hypermutation, leading to the view that pulmonary MALT lymphomas and HIV-negative LIP are under the influence of germinal center hypermutation mechanisms. Because BCL-6 mutations in HIV-related LIP cases did not demonstrate features of Ig VH gene hypermutation, immunological reactions in HIV-related LIP are the result of a process different from that found in HIV-negative pulmonary lymphoproliferative disorders.
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U2 - 10.4049/jimmunol.172.11.7116
DO - 10.4049/jimmunol.172.11.7116
M3 - Article
C2 - 15153535
AN - SCOPUS:2442641494
VL - 172
SP - 7116
EP - 7122
JO - Journal of Immunology
JF - Journal of Immunology
SN - 0022-1767
IS - 11
ER -