Base composition of terminal polynucleotides isolated from mouse ascite nucleolar RNA

John C. Lee

Research output: Contribution to journalArticlepeer-review

Abstract

Two species of high molecular weight RNA were isolated from the nucleoli of mouse ascite tumor cells. The 3′-terminal nucleotide fragments of these high molecular weight RNA species were obtained after digestion of the RNA with RNase T1, oxidation of the terminus with sodium periodate and isolation on aminoethyl cellulose. The terminal fragments behaved differently upon chromatography on DEAE-cellulose. These fragments contained different base compositions as follows: fragment A - Ap 24.7%, Cp 50.3%, and Up 25.0%; fragment B - Ap 40.7%, Cp 16.8%, and Up 43.5%. The terminal polynucleotide sequences of the 41S and 45S RNAs are -Gp(Ap, Up, 2Cp)XOH and -Gp(2Ap, 2Up, Cp)XOH respectively where X is unknown at the present. These data are discussed in regard to the various models proposed for the organization of nucleotide sequences of rRNA within the nucleolar precursors.

Original languageEnglish (US)
Pages (from-to)1257-1262
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume66
Issue number4
DOIs
StatePublished - Oct 27 1975

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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