BAL is a novel risk-related gene in diffuse large B-cell lymphomas that enhances cellular migration

Ricardo C.T. Aguiar, Yoshihiro Yakushijin, Samir Kharbanda, Ravi Salgia, Jonathan A. Fletcher, Margaret A. Shipp

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97 Scopus citations

Abstract

Clinical risk factor models such as the International Prognostic Index are used to Identify diffuse large B-cell lymphoma (DLB-CL) patients with different risks of death from their diseases. To elucidate the molecular bases for these observed clinical differences in outcome, differential display was used to identify a novel gene, termed BAL (B-aggressive lymphoma), which is expressed at significantly higher levels in fatal high-risk DLB-CLs than in cured low-risk tumors. The major BAL complementary DNA encodes a previously uncharacterized 88-kd nuclear protein with a duplicated N-terminal domain homologous to the nonhistone portion of histone-macroH2A and a C-terminal alpha-helical region with 2 short coiled-coil domains. Of note, the BAL N-terminus and secondary structure resemble those of a recently identified human protein, KIAA1268. In addition, both BAL and KIAA1268 map to chromosome 3q21, further suggesting that these genes belong to a newly identified family. BAL is expressed at increased levels in DLB-CL cell lines with an activated peripheral B cell, rather than a germinal center B cell, phenotype. This observation and the characteristic dissemination of high risk DLB-CLs prompted studies regarding the role of BAL in B-cell migration. In classical transwell assays, stable BAL-overexpressing B-cell lymphoma transfectants had significantly higher rates of migration than vector-only transfectants, indicating that the risk-related BAL gene pro-motes malignant B-cell migration.

Original languageEnglish (US)
Pages (from-to)4328-4334
Number of pages7
JournalBlood
Volume96
Issue number13
StatePublished - Dec 1 2000
Externally publishedYes

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ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

Cite this

Aguiar, R. C. T., Yakushijin, Y., Kharbanda, S., Salgia, R., Fletcher, J. A., & Shipp, M. A. (2000). BAL is a novel risk-related gene in diffuse large B-cell lymphomas that enhances cellular migration. Blood, 96(13), 4328-4334.