Baculovirus vector-mediated expression of heterologous genes in insect cells

P. Sridhar, A. K. Awasthi, A. A. Azim, S. Burma, S. Habib, A. Jain, B. Mukherjee, A. Ranjan, Seyed E. Hasnain

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

The baculovirus expression system employing Autagrapha californica nuclear polyhidrosis virus and Spodoptera frugiperda insect cells in culture has proved very popular for high level expression of heterologous genes: In this system, transcription of the foreign gene is usually driven by the hyperactive and temporally regulated polyhedrin gene promoter. Replacement of the polyhedrin gene, which encodes a 29-kDa occlusion protein (non-essential for viral replication), with a gene of interest leads to an occlusion negative phenotype which serves as a visual marker to select for recombinant viruses. Simultaneous expression of multiple genes can also be achieved. The heterologous proteins synthesized in this system are antigenically, immunologically and functionally identical in most respects to their native counterparts. This mini-review will aim at summarizing the potentials and utility of the baculovirus expression vector system and will address some important questions relating to the biology of this system.

Original languageEnglish (US)
Pages (from-to)603-614
Number of pages12
JournalJ Biosci
Volume19
Issue number5
DOIs
StatePublished - Dec 1994

Keywords

  • Baculovirus
  • Spodoptera frugiperda
  • heterologous gene
  • insect cells
  • utagrapha californica

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

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  • Cite this

    Sridhar, P., Awasthi, A. K., Azim, A. A., Burma, S., Habib, S., Jain, A., Mukherjee, B., Ranjan, A., & Hasnain, S. E. (1994). Baculovirus vector-mediated expression of heterologous genes in insect cells. J Biosci, 19(5), 603-614. https://doi.org/10.1007/BF02703205