Bacteriophages and endotoxin in licensed live virus vaccines

E. E.M. Moody; M. D. Trousdale; J. H. Jorgensen; A. Shelokov

doi:10.1093/infdis/131.5.588

Bacteriophages and endotoxin in licensed live virus vaccines

E. E.M. Moody, M. D. Trousdale, J. H. Jorgensen, A. Shelokov

Pathology

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

In confirmation of recent reports, coliphages were found in seven of 19 unselected samples of the currently licensed live virus vaccines. Coliphages and pseudomonas phages were found in 11 and 14, respectively, of the 20 bovine sera commonly used in the cell culture phase of virus vaccine production. The same lots of vaccine and serum were examined by the limulus assay for endotoxin, another product of bacterial contamination. Eighteen of 20 sera had detectable endotoxin like activity. These preliminary results suggest that endotoxin activity may serve as a sensitive indicator of residual products of previous bacterial contamination, including bacteriophages.

Original language	English (US)
Pages (from-to)	588-591
Number of pages	4
Journal	Journal of Infectious Diseases
Volume	131
Issue number	5
DOIs	https://doi.org/10.1093/infdis/131.5.588
State	Published - 1975

ASJC Scopus subject areas

Immunology and Allergy
Infectious Diseases

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title = "Bacteriophages and endotoxin in licensed live virus vaccines",

abstract = "In confirmation of recent reports, coliphages were found in seven of 19 unselected samples of the currently licensed live virus vaccines. Coliphages and pseudomonas phages were found in 11 and 14, respectively, of the 20 bovine sera commonly used in the cell culture phase of virus vaccine production. The same lots of vaccine and serum were examined by the limulus assay for endotoxin, another product of bacterial contamination. Eighteen of 20 sera had detectable endotoxin like activity. These preliminary results suggest that endotoxin activity may serve as a sensitive indicator of residual products of previous bacterial contamination, including bacteriophages.",

author = "Moody, {E. E.M.} and Trousdale, {M. D.} and Jorgensen, {J. H.} and A. Shelokov",

note = "Funding Information: This work was supported by grants no. 5-S01-RR-05654-05 from the National Institutes of Health (General Research Support) and no. 3-3 from the State of Texas Organized Research.",

year = "1975",

doi = "10.1093/infdis/131.5.588",

language = "English (US)",

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T1 - Bacteriophages and endotoxin in licensed live virus vaccines

AU - Moody, E. E.M.

AU - Trousdale, M. D.

AU - Jorgensen, J. H.

AU - Shelokov, A.

N1 - Funding Information: This work was supported by grants no. 5-S01-RR-05654-05 from the National Institutes of Health (General Research Support) and no. 3-3 from the State of Texas Organized Research.

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N2 - In confirmation of recent reports, coliphages were found in seven of 19 unselected samples of the currently licensed live virus vaccines. Coliphages and pseudomonas phages were found in 11 and 14, respectively, of the 20 bovine sera commonly used in the cell culture phase of virus vaccine production. The same lots of vaccine and serum were examined by the limulus assay for endotoxin, another product of bacterial contamination. Eighteen of 20 sera had detectable endotoxin like activity. These preliminary results suggest that endotoxin activity may serve as a sensitive indicator of residual products of previous bacterial contamination, including bacteriophages.

AB - In confirmation of recent reports, coliphages were found in seven of 19 unselected samples of the currently licensed live virus vaccines. Coliphages and pseudomonas phages were found in 11 and 14, respectively, of the 20 bovine sera commonly used in the cell culture phase of virus vaccine production. The same lots of vaccine and serum were examined by the limulus assay for endotoxin, another product of bacterial contamination. Eighteen of 20 sera had detectable endotoxin like activity. These preliminary results suggest that endotoxin activity may serve as a sensitive indicator of residual products of previous bacterial contamination, including bacteriophages.

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Bacteriophages and endotoxin in licensed live virus vaccines

Abstract

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