Bacteriophages and endotoxin in licensed live virus vaccines

E. E.M. Moody; M. D. Trousdale; J. H. Jorgensen; A. Shelokov

doi:10.1093/infdis/131.5.588

Bacteriophages and endotoxin in licensed live virus vaccines

E. E.M. Moody, M. D. Trousdale, J. H. Jorgensen, A. Shelokov

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

In confirmation of recent reports, coliphages were found in seven of 19 unselected samples of the currently licensed live virus vaccines. Coliphages and pseudomonas phages were found in 11 and 14, respectively, of the 20 bovine sera commonly used in the cell culture phase of virus vaccine production. The same lots of vaccine and serum were examined by the limulus assay for endotoxin, another product of bacterial contamination. Eighteen of 20 sera had detectable endotoxin like activity. These preliminary results suggest that endotoxin activity may serve as a sensitive indicator of residual products of previous bacterial contamination, including bacteriophages.

Original language	English (US)
Pages (from-to)	588-591
Number of pages	4
Journal	Journal of Infectious Diseases
Volume	131
Issue number	5
DOIs	https://doi.org/10.1093/infdis/131.5.588
State	Published - 1975
Externally published	Yes

ASJC Scopus subject areas

Immunology and Allergy
Infectious Diseases

Access to Document

10.1093/infdis/131.5.588

Cite this

@article{5255a9a886a74c82a22413a6a6e620f8,

title = "Bacteriophages and endotoxin in licensed live virus vaccines",

abstract = "In confirmation of recent reports, coliphages were found in seven of 19 unselected samples of the currently licensed live virus vaccines. Coliphages and pseudomonas phages were found in 11 and 14, respectively, of the 20 bovine sera commonly used in the cell culture phase of virus vaccine production. The same lots of vaccine and serum were examined by the limulus assay for endotoxin, another product of bacterial contamination. Eighteen of 20 sera had detectable endotoxin like activity. These preliminary results suggest that endotoxin activity may serve as a sensitive indicator of residual products of previous bacterial contamination, including bacteriophages.",

author = "Moody, {E. E.M.} and Trousdale, {M. D.} and Jorgensen, {J. H.} and A. Shelokov",

note = "Funding Information: This work was supported by grants no. 5-S01-RR-05654-05 from the National Institutes of Health (General Research Support) and no. 3-3 from the State of Texas Organized Research.",

year = "1975",

doi = "10.1093/infdis/131.5.588",

language = "English (US)",

volume = "131",

pages = "588--591",

journal = "Journal of Infectious Diseases",

issn = "0022-1899",

publisher = "Oxford University Press",

number = "5",

}

TY - JOUR

T1 - Bacteriophages and endotoxin in licensed live virus vaccines

AU - Moody, E. E.M.

AU - Trousdale, M. D.

AU - Jorgensen, J. H.

AU - Shelokov, A.

N1 - Funding Information: This work was supported by grants no. 5-S01-RR-05654-05 from the National Institutes of Health (General Research Support) and no. 3-3 from the State of Texas Organized Research.

PY - 1975

Y1 - 1975

N2 - In confirmation of recent reports, coliphages were found in seven of 19 unselected samples of the currently licensed live virus vaccines. Coliphages and pseudomonas phages were found in 11 and 14, respectively, of the 20 bovine sera commonly used in the cell culture phase of virus vaccine production. The same lots of vaccine and serum were examined by the limulus assay for endotoxin, another product of bacterial contamination. Eighteen of 20 sera had detectable endotoxin like activity. These preliminary results suggest that endotoxin activity may serve as a sensitive indicator of residual products of previous bacterial contamination, including bacteriophages.

AB - In confirmation of recent reports, coliphages were found in seven of 19 unselected samples of the currently licensed live virus vaccines. Coliphages and pseudomonas phages were found in 11 and 14, respectively, of the 20 bovine sera commonly used in the cell culture phase of virus vaccine production. The same lots of vaccine and serum were examined by the limulus assay for endotoxin, another product of bacterial contamination. Eighteen of 20 sera had detectable endotoxin like activity. These preliminary results suggest that endotoxin activity may serve as a sensitive indicator of residual products of previous bacterial contamination, including bacteriophages.

UR - http://www.scopus.com/inward/record.url?scp=0016822487&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0016822487&partnerID=8YFLogxK

U2 - 10.1093/infdis/131.5.588

DO - 10.1093/infdis/131.5.588

M3 - Article

C2 - 805187

AN - SCOPUS:0016822487

VL - 131

SP - 588

EP - 591

JO - Journal of Infectious Diseases

JF - Journal of Infectious Diseases

SN - 0022-1899

IS - 5

ER -

Bacteriophages and endotoxin in licensed live virus vaccines

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this