Bacteriophage P22 capsids with a subgenome length of packaged DNA

P. Serwer, R. Gope

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Bacteriophage P22 assembles a DNA-free procapsid that subsequently packages P22 DNA. To study the packaging of bacteriophage P22 DNA, attempts were made to isolate P22 capsids with a subgenome length of packaged DNA. With the use of cesium chloride buoyant density sedimentation and agarose gel electrophoresis, the following capsids with a subgenome length of packaged DNA were isolated and characterized: (i) a capsid with the solid-support-free electrophoretic mobility and radius of the DNA-free P22 procapsid; (ii) a capsid with the solid-support-free electrophoretic mobility and radius of the mature P22 bacteriophage; and (iii) a capsid with a solid-support-free electrophoretic mobility and possibly a radius intermediate to those of the procapsid and bacteriophage.

Original languageEnglish (US)
Pages (from-to)293-296
Number of pages4
JournalJournal of virology
Volume49
Issue number1
DOIs
StatePublished - 1984

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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