Abstract
Bacteriophage P22 assembles a DNA-free procapsid that subsequently packages P22 DNA. To study the packaging of bacteriophage P22 DNA, attempts were made to isolate P22 capsids with a subgenome length of packaged DNA. With the use of cesium chloride buoyant density sedimentation and agarose gel electrophoresis, the following capsids with a subgenome length of packaged DNA were isolated and characterized: (i) a capsid with the solid-support-free electrophoretic mobility and radius of the DNA-free P22 procapsid; (ii) a capsid with the solid-support-free electrophoretic mobility and radius of the mature P22 bacteriophage; and (iii) a capsid with a solid-support-free electrophoretic mobility and possibly a radius intermediate to those of the procapsid and bacteriophage.
Original language | English (US) |
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Pages (from-to) | 293-296 |
Number of pages | 4 |
Journal | Journal of virology |
Volume | 49 |
Issue number | 1 |
DOIs | |
State | Published - 1984 |
ASJC Scopus subject areas
- Microbiology
- Immunology
- Insect Science
- Virology