Bacterial lipopolysaccharide-mediated murine fetal death: The role of interleukin-1

R. M. Silver, S. S. Edwin, F. Umar, D. J. Dudley, D. W. Branch, M. D. Mitchell

Research output: Contribution to journalArticlepeer-review

33 Scopus citations

Abstract

OBJECTIVE: Our purpose was to determine whether interleukin-1 is an important mediator of lipopolysaccharide-induced fetal death and, if so, whether interleukin-1 causes fetal death by inducing prostanoid formation in gestational tissues. STUDY DESIGN: Pregnant C3H/HeN mice were administered lipopolysaccharide, interleukin-1α, interleukin-β, or vehicle on days 11 to 13 of pregnancy. Mice were killed 72 hours later and the fetal status was determined. Some mice were pretreated with anti-interleukin-1-receptor antibodies or indomethacin. Decidual explants were established from treated mice, and supernatants were assayed for interleukin-1β and prostaglandin E2. RESULTS: Decidua taken from lipopolysaccharide-treated mice produced significantly increased amounts of interleukin-1β, and pretreatment with anti-interleukin-1-receptor antibodies reduced the proportion of fetal deaths after lipopolysaccharide administration from 100% to 33%. The administration of interleukin-1α caused fetal death in a dose-dependent fashion, and decidua taken from interleukin-1-treated mice produced significantly increased amounts of prostaglandin E2. However, pretreatment with doses of indomethacin that abrogated decidual prostaglandin E2 production did not reduce the proportion of fetal death after interleukin-1α administration. CONCLUSIONS: Interleukin-1 is an important mediator of lipopolysaccharide- induced fetal death and causes fetal death by prostaglandin-independent effects.

Original languageEnglish (US)
Pages (from-to)544-549
Number of pages6
JournalAmerican Journal of Obstetrics and Gynecology
Volume176
Issue number3
DOIs
StatePublished - 1997
Externally publishedYes

Keywords

  • fetal death
  • interleukin-1
  • Lipopolysaccharide
  • prostaglandins

ASJC Scopus subject areas

  • Obstetrics and Gynecology

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