B cells with high side scatter parameter by flow cytometry correlate with inferior survival in diffuse large B-cell lymphoma

Ali Bashashati, Nathalie A. Johnson, Alireza Hadj Khodabakhshi, Matthew D. Whiteside, Habil Zare, David W. Scott, Kenneth Lo, Raphael Gottardo, Fiona S.L. Brinkman, Joseph M. Connors, Graham W. Slack, Randy D. Gascoyne, Andrew P. Weng, Ryan R. Brinkman

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Despite advances in the understanding of diffuse large B-cell lymphoma (DLBCL) biology, only the clinically based International Prognostic Index (IPI) is used routinely for risk stratification at diagnosis. To find novel prognostic markers, we analyzed flow cytometric data from 229 diagnostic DLBCL samples using an automated multiparameter data analysis approach developed in our laboratory. By using the developed automated data analysis pipeline, we identified 71 of 229 cases as having more than 35% B cells with a high side scatter (SSC) profile, a parameter reflecting internal cellular complexity. This high SSC B-cell feature was associated with inferior overall and progression-free survival (P = .001 and P = .01, respectively) and remained a significant predictor of overall survival in multivariate Cox regression analysis (IPI, P = .001; high SSC, P = .004; rituximab, P = .53). This study suggests that high SSC among B cells may serve as a useful biomarker to identify patients with DLBCL at high risk for relapse. This is of particular interest because this biomarker is readily available in most clinical laboratories without significant alteration to existing routine diagnostic strategies or incurring additional costs.

Original languageEnglish (US)
Pages (from-to)805-814
Number of pages10
JournalAmerican journal of clinical pathology
Volume137
Issue number5
DOIs
StatePublished - May 2012
Externally publishedYes

Keywords

  • Diffuse large B-cell lymphoma
  • Flow cytometry
  • Lymphoma
  • Side scatter
  • Survival

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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