TY - JOUR
T1 - B cell subpopulations separated by CD27 and crucial collaboration of CD27 + B cells and helper T cells in immunoglobulin production
AU - Agematsu, Kazunaga
AU - Nagumo, Haruo
AU - Yang, Fen Chun
AU - Nakazawa, Takayuki
AU - Fukushima, Keitaro
AU - Ito, Susumu
AU - Sugita, Kanji
AU - Mori, Tetsuo
AU - Kobata, Tetsuji
AU - Morimoto, Chikao
AU - Komiyama, Atsushi
PY - 1997/8
Y1 - 1997/8
N2 - B cell immunoglobulin production is regulated by helper T cells through direct interaction and secreted cytokines. In the present study, we functionally analyzed CD27 in cord and peripheral blood B cells. Adult peripheral blood B cells were separated into CD27 + and CD27 - cells, which differed in their morphology. Cord blood B cells did not express CD27, and CD27 expression on peripheral blood B cells increased with age. Only CD27 + B cells had the ability to produce immunoglobulin, which was increased by contact with a tumor necrosis factor-related transmembrane ligand, CD70. Adult peripheral blood CD27 + B cells can be further subdivided into two discrete subtypes: IgD- CD27 + and IgD + CD27 + B cells. IgD- CD27 + B cells produce IgG, IgM and IgA, whereas IgD + CD27 + B cells predominantly produce IgM. The addition of activated CD4 +CD45RO T cells expressing CD70 caused down-regulation of CD27 expression on activated B cells, and this down-modulation was completely blocked by anti-CD70 monoclonal antibody, indicating direct T-B cell contact via CD27/CD70. The triggering via CD27 and CD40 additively increased the immunoglobulin production under Staphylococcus aureus Cowan strain plus interleukin-2 stimulation. Taken together, our findings demonstrate that peripheral blood B cells are separated into subpopulations by CD27 and IgD expression and that CD27 + B cells produce large amounts of immunoglobulin by interaction with the CD70 molecule.
AB - B cell immunoglobulin production is regulated by helper T cells through direct interaction and secreted cytokines. In the present study, we functionally analyzed CD27 in cord and peripheral blood B cells. Adult peripheral blood B cells were separated into CD27 + and CD27 - cells, which differed in their morphology. Cord blood B cells did not express CD27, and CD27 expression on peripheral blood B cells increased with age. Only CD27 + B cells had the ability to produce immunoglobulin, which was increased by contact with a tumor necrosis factor-related transmembrane ligand, CD70. Adult peripheral blood CD27 + B cells can be further subdivided into two discrete subtypes: IgD- CD27 + and IgD + CD27 + B cells. IgD- CD27 + B cells produce IgG, IgM and IgA, whereas IgD + CD27 + B cells predominantly produce IgM. The addition of activated CD4 +CD45RO T cells expressing CD70 caused down-regulation of CD27 expression on activated B cells, and this down-modulation was completely blocked by anti-CD70 monoclonal antibody, indicating direct T-B cell contact via CD27/CD70. The triggering via CD27 and CD40 additively increased the immunoglobulin production under Staphylococcus aureus Cowan strain plus interleukin-2 stimulation. Taken together, our findings demonstrate that peripheral blood B cells are separated into subpopulations by CD27 and IgD expression and that CD27 + B cells produce large amounts of immunoglobulin by interaction with the CD70 molecule.
KW - CD27
KW - Human
KW - IgD
KW - T cell-B cell collaboration
UR - http://www.scopus.com/inward/record.url?scp=0030786425&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030786425&partnerID=8YFLogxK
U2 - 10.1002/eji.1830270835
DO - 10.1002/eji.1830270835
M3 - Article
C2 - 9295047
AN - SCOPUS:0030786425
SN - 0014-2980
VL - 27
SP - 2073
EP - 2079
JO - European Journal of Immunology
JF - European Journal of Immunology
IS - 8
ER -