Abstract
The objective of this study was to develop a more effective liposome-based method for delivering drugs to mediastinal nodes. Nodal uptake was determined after intrapleural injection of the avidin/biotin-liposome system in normal rats. The effect of injection sequence (avidin injected 2 h before biotin-liposomes and vice versa), volume injected, and administered dose of the agents is described. Pharmacokinetics of the avidin/biotin-liposome system was monitored with scintigraphic imaging by labeling the biotin-liposomes with technetium-99m (99mTc). To identify the nodes during the biodistribution studies, patent blue dye was encapsulated in the biotin-liposomes. Tissue biodistribution studies were performed 22 h after injection of the 99mTc-blue-biotin-liposomes. When avidin was injected before 99mTc-blue-biotin-liposomes, better mediastinal node targeting (15.7%; p < 0.05) was achieved than when biotin-liposomes were injected first (8.3%) or when only biotin-liposomes were injected (1.0%). Injection of a small dose of liposomes (0.5 mg phospholipid) and avidin (0.5 mg) resulted in the most favorable drug delivery to mediastinal nodes and other organs. Intrapleural injection of the avidin/biotin-liposome system could potentially be used for drug delivery to disease processes such as lung cancer, anthrax, and tuberculosis that invade mediastinal nodes and use them as centers of incubation and dissemination.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2595-2608 |
| Number of pages | 14 |
| Journal | Journal of Pharmaceutical Sciences |
| Volume | 93 |
| Issue number | 10 |
| DOIs | |
| State | Published - Oct 2004 |
Fingerprint
Keywords
- Drug targeting
- Liposomes
- Lymphatic transport
- Pharmacokinetics
- Scintigraphy
ASJC Scopus subject areas
- Drug Discovery
- Organic Chemistry
- Chemistry(all)
- Molecular Medicine
- Pharmacology
- Pharmaceutical Science
Cite this
Avidin/biotin-liposome system injected in the pleural space for drug delivery to mediastinal lymph nodes. / Medina, Luis A.; Calixto, Sergio M.; Klipper, Robert; Phillips, William T; Goins, Beth.
In: Journal of Pharmaceutical Sciences, Vol. 93, No. 10, 10.2004, p. 2595-2608.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Avidin/biotin-liposome system injected in the pleural space for drug delivery to mediastinal lymph nodes
AU - Medina, Luis A.
AU - Calixto, Sergio M.
AU - Klipper, Robert
AU - Phillips, William T
AU - Goins, Beth
PY - 2004/10
Y1 - 2004/10
N2 - The objective of this study was to develop a more effective liposome-based method for delivering drugs to mediastinal nodes. Nodal uptake was determined after intrapleural injection of the avidin/biotin-liposome system in normal rats. The effect of injection sequence (avidin injected 2 h before biotin-liposomes and vice versa), volume injected, and administered dose of the agents is described. Pharmacokinetics of the avidin/biotin-liposome system was monitored with scintigraphic imaging by labeling the biotin-liposomes with technetium-99m (99mTc). To identify the nodes during the biodistribution studies, patent blue dye was encapsulated in the biotin-liposomes. Tissue biodistribution studies were performed 22 h after injection of the 99mTc-blue-biotin-liposomes. When avidin was injected before 99mTc-blue-biotin-liposomes, better mediastinal node targeting (15.7%; p < 0.05) was achieved than when biotin-liposomes were injected first (8.3%) or when only biotin-liposomes were injected (1.0%). Injection of a small dose of liposomes (0.5 mg phospholipid) and avidin (0.5 mg) resulted in the most favorable drug delivery to mediastinal nodes and other organs. Intrapleural injection of the avidin/biotin-liposome system could potentially be used for drug delivery to disease processes such as lung cancer, anthrax, and tuberculosis that invade mediastinal nodes and use them as centers of incubation and dissemination.
AB - The objective of this study was to develop a more effective liposome-based method for delivering drugs to mediastinal nodes. Nodal uptake was determined after intrapleural injection of the avidin/biotin-liposome system in normal rats. The effect of injection sequence (avidin injected 2 h before biotin-liposomes and vice versa), volume injected, and administered dose of the agents is described. Pharmacokinetics of the avidin/biotin-liposome system was monitored with scintigraphic imaging by labeling the biotin-liposomes with technetium-99m (99mTc). To identify the nodes during the biodistribution studies, patent blue dye was encapsulated in the biotin-liposomes. Tissue biodistribution studies were performed 22 h after injection of the 99mTc-blue-biotin-liposomes. When avidin was injected before 99mTc-blue-biotin-liposomes, better mediastinal node targeting (15.7%; p < 0.05) was achieved than when biotin-liposomes were injected first (8.3%) or when only biotin-liposomes were injected (1.0%). Injection of a small dose of liposomes (0.5 mg phospholipid) and avidin (0.5 mg) resulted in the most favorable drug delivery to mediastinal nodes and other organs. Intrapleural injection of the avidin/biotin-liposome system could potentially be used for drug delivery to disease processes such as lung cancer, anthrax, and tuberculosis that invade mediastinal nodes and use them as centers of incubation and dissemination.
KW - Drug targeting
KW - Liposomes
KW - Lymphatic transport
KW - Pharmacokinetics
KW - Scintigraphy
UR - http://www.scopus.com/inward/record.url?scp=4844229630&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=4844229630&partnerID=8YFLogxK
U2 - 10.1002/jps.20163
DO - 10.1002/jps.20163
M3 - Article
C2 - 15349969
AN - SCOPUS:4844229630
VL - 93
SP - 2595
EP - 2608
JO - Journal of Pharmaceutical Sciences
JF - Journal of Pharmaceutical Sciences
SN - 0022-3549
IS - 10
ER -