Autoradiographic and biochemical studies on hormone receptor localization

P. J. Sheridan, J. M. Buchanan, V. Anselmo

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Autoradiography has been used in the past to locate presumptive receptor systems for a number of steroids. It has provided information in rather complex tissues that could not have been obtained by biochemical procedures. In these studies we made use of autoradiography to redirect our biochemical efforts to study androgen receptors in the primate heart. Castrated-adrenalectomized female rhesus monkeys and baboons were injected with 1 μg of 5 α-dihydro-[1,2,4,5,6,7-3H]-testosterone per kilogram of body weight. The animals were killed 1 hour later and the hearts were removed and processed for autoradiography. A nuclear localization of androgen was found in most of the myocardial cells, but in few if any of the interstitial cells. After reviewing the autoradiographic data, we began a new series of biochemical studies using a new buffer system and unlabeled androgen to stabilize the receptor. This in combination with postlabeled gradients allowed us to demonstrate for the first time 8S binding on sucrose density gradients. We feel that autoradiography can be a useful adjunct to biochemical studies even in 'less complex' tissue such as the cardiac muscle.

Original languageEnglish (US)
Pages (from-to)195-200
Number of pages6
JournalJournal of Histochemistry and Cytochemistry
Volume29
Issue number1 SUPPL.
DOIs
StatePublished - 1981
Externally publishedYes

ASJC Scopus subject areas

  • Anatomy
  • Histology

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