Autonomous regulation of sex-specific developmental programming in mouse fetal germ cells

Kazuhiro Iwahashi, Hirotaka Yoshioka, Eleanor W. Low, John R. McCarrey, Ryuzo Yanagimachi, Yukiko Yamazaki

Research output: Contribution to journalArticlepeer-review

16 Scopus citations


In mice, unique events regulating epigenetic programming (e.g., genomic imprinting) and replication state (mitosis versus meiosis) occur during fetal germ cell development. To determine whether these processes are autonomously programmed in fetal germ cells or are dependent upon ongoing instructive interactions with surrounding gonadal somatic cells, we isolated male and female germ cells at 13.5 days postcoitum (dpc) and maintained them in culture for 6 days, either alone or in the presence of feeder cells or gonadal somatic cells. We examined allele-specific DNA methylation in the imprinted H19 and Snrpn genes, and we also determined whether these cells remained mitotic or entered meiosis. Our results show that isolated male germ cells are able to establish a characteristic "paternal" methylation pattern at imprinted genes in the absence of any support from somatic cells. On the other hand, cultured female germ cells maintain a hypomethylated status at these loci, characteristic of the normal "maternal" methylation pattern in endogenous female germ cells before birth. Further, the surviving female germ cells entered first meiotic prophase and reached the pachytene stage, whereas male germ cells entered mitotic arrest. These results indicate that mechanisms controlling both epigenetic programming and replication state are autonomously regulated in fetal germ cells that have been exposed to the genital ridge prior to 13.5 dpc.

Original languageEnglish (US)
Pages (from-to)697-706
Number of pages10
JournalBiology of reproduction
Issue number4
StatePublished - Oct 2007
Externally publishedYes


  • De novo methylation
  • Genomic imprinting
  • Germ cells
  • Meiosis
  • Sex differentiation

ASJC Scopus subject areas

  • Reproductive Medicine
  • Cell Biology


Dive into the research topics of 'Autonomous regulation of sex-specific developmental programming in mouse fetal germ cells'. Together they form a unique fingerprint.

Cite this