TY - JOUR
T1 - Autologous human cellular cytotoxicity induced by mitogenic and nonmitogenic lectins
AU - MacDermott, R. P.
AU - Nash, G. S.
AU - Saint, J. G.
AU - Clark, E. A.
AU - Zaras, A. G.
AU - Boldt, D.
PY - 1976/12/1
Y1 - 1976/12/1
N2 - Autologous systems are of great interest in the study of cellular cytotoxicity. By eliminating considerations of previous allogeneic sensitization, an autologous system allows straight forward study of effector mechanisms. The induction of lymphocytotoxicity by a variety of plant lectins toward xenogeneic, allogeneic, or syngeneic target cells has been described. Although early studies of lectin induced lymphocytotoxicity demonstrated a close correlation between the degree of mitogenic stimulation of the lymphocytes and subsequent cytotoxicity, recent work has suggested that the phenomena of mitogenesis and of cytoxocity may be independent of one another. The ability to fractionate phytohemagglutinin into its major components, and the existence of other plant lectins that are nonmitogenic, provide an opportunity to study human autologous cellular cytotoxicity induced by lectins, with particular attention directed to the relationship between cytotoxicity and mitogenicity. In this investigation, the authors have compared the ability of the mitogenic components of phytohemagglutinin (PHA) and the nonmitogenic lectin, wheat germ agglutinin (WGA), to cause autologous cellular cytotoxicity of human red blood cells (RBC).
AB - Autologous systems are of great interest in the study of cellular cytotoxicity. By eliminating considerations of previous allogeneic sensitization, an autologous system allows straight forward study of effector mechanisms. The induction of lymphocytotoxicity by a variety of plant lectins toward xenogeneic, allogeneic, or syngeneic target cells has been described. Although early studies of lectin induced lymphocytotoxicity demonstrated a close correlation between the degree of mitogenic stimulation of the lymphocytes and subsequent cytotoxicity, recent work has suggested that the phenomena of mitogenesis and of cytoxocity may be independent of one another. The ability to fractionate phytohemagglutinin into its major components, and the existence of other plant lectins that are nonmitogenic, provide an opportunity to study human autologous cellular cytotoxicity induced by lectins, with particular attention directed to the relationship between cytotoxicity and mitogenicity. In this investigation, the authors have compared the ability of the mitogenic components of phytohemagglutinin (PHA) and the nonmitogenic lectin, wheat germ agglutinin (WGA), to cause autologous cellular cytotoxicity of human red blood cells (RBC).
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M3 - Article
C2 - 977956
AN - SCOPUS:0017176414
VL - 117
SP - 1402
EP - 1403
JO - Journal of Immunology
JF - Journal of Immunology
SN - 0022-1767
IS - 4
ER -