In the version of this Article originally published, several images in Fig. 6e and g were mislabeled. “GFP shRNA” should be added to all the panels in Fig. 6e, and “DMSO” should be added to all panels in Fig. 6g. The legend should also be updated accordingly as: “e, HCA2 cells were infected with a control lentivirus (LP3) or lentivirus carrying oncogenic RAS. Cells were cultured with vehicle (DMSO) for 10 days or drug (Rapa) for 3 days (acute) and switched to DMSO for an additional 7 days or continuously treated (chronic) for 10 days with Rapa. Thereafter, clonogenic assays were performed. f, HCA2 cells were co-infected with RAS and lentiviruses carrying shRNAs to deplete the indicated proteins. Transcript levels (relative to GFP shRNA) for IL6 were quantified by qPCR. g, The DMSO-treated cells described in e were additionally infected (co-infected) with shRNAs to deplete the indicated proteins; 10 days later, clonogenic assays were performed as described in e. h, HCA2 cells infected with lentiviruses carrying the indicated shRNAs were induced to senesce by ionizing radiation or were mock irradiated (NS, non-senescent). Clonogenic assays were performed 10 days later.”.
ASJC Scopus subject areas
- Cell Biology