TY - JOUR
T1 - Attenuation of the natural course of herpes simplex virus infection in human oral epithelial cell cultures by smokeless tobacco extracts suggests the possibility of a synergistic mechanism for carcinogenesis
AU - Murrah, Valerie A.
AU - Gilchrist, Eric P.
AU - Moyer, Mary Pat
N1 - Funding Information:
Supported by American Cancer Society Clinical Oncology Career Development Award (ACS 90-220), American Cancer Society Institutional Research Grant (#IRG-116L), and Smokeless Tobacco Research Council Grants (#0200 and #0528). Presented in abstract form at the 1992, 47th annual meeting of the American Academy of Oral Pathology. aAssociate Professor, Department of Pathology and Laboratory Medicine, Emory University School of Medicine. bResearch Associate, Department of Pathology and Laboratory Medicine, Emory University School of Medicine. cprofessor, Department of Surgery, The University of Texas Health Science Center. Received for publication Jan. 16, 1995; returned for revision Feb. 22, 1995; accepted for publication Aug. 1, 1995. Copyright 9 1996 by Mosby-Year Book, Inc. 1079-2104/96/$5.00 + 0 7/14/68645
PY - 1996
Y1 - 1996
N2 - High prevalence of both tobacco use and latent herpes simplex virus type 1 suggests the opportunity for synergism between these agents as cocarcinogens. In this study, postprimary human oral epithelial cell cultures were infected with herpes simplex virus type 1 pretreated with 2% extracts of either loose leaf, moist, or dry snuffs. Cultures were subsequently periodically exposed to the tobacco. Parameters measured included percentage of cultures undergoing active virus production, onset and time course of cytopathic effects, and concentration of virus released into the media over time. Results showed inhibition of both herpes simplex virus-mediated cell lysis and viral replication by tobacco extracts. This is the first time that these phenomena have been demonstrated in normal human oral epithelial cells. The work described here provides evidence to support a hypothesis that herpes simplex virus type 1 and smokeless tobacco may act synergistically in oral carcinogenesis.
AB - High prevalence of both tobacco use and latent herpes simplex virus type 1 suggests the opportunity for synergism between these agents as cocarcinogens. In this study, postprimary human oral epithelial cell cultures were infected with herpes simplex virus type 1 pretreated with 2% extracts of either loose leaf, moist, or dry snuffs. Cultures were subsequently periodically exposed to the tobacco. Parameters measured included percentage of cultures undergoing active virus production, onset and time course of cytopathic effects, and concentration of virus released into the media over time. Results showed inhibition of both herpes simplex virus-mediated cell lysis and viral replication by tobacco extracts. This is the first time that these phenomena have been demonstrated in normal human oral epithelial cells. The work described here provides evidence to support a hypothesis that herpes simplex virus type 1 and smokeless tobacco may act synergistically in oral carcinogenesis.
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U2 - 10.1016/S1079-2104(96)80150-8
DO - 10.1016/S1079-2104(96)80150-8
M3 - Article
C2 - 8850486
AN - SCOPUS:0029678431
SN - 1079-2104
VL - 81
SP - 63
EP - 69
JO - Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics
JF - Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics
IS - 1
ER -