Attenuation of intestinal ischemia/reperfusion injury with sodium nitroprusside: Studies on mitochondrial function and lipid changes

Muniswamy Madesh, Anup Ramachandran, Anna Pulimood, Markandeyulu Vadranam, K. A. Balasubramanian

Research output: Contribution to journalArticlepeer-review

31 Scopus citations

Abstract

Reactive oxygen species have been implicated in cellular injury during ischemia/reperfusion (I/R). Mitochondria are one of the main targets of oxygen free radicals and damage to this organelle leads to cell death. Reports suggest that nitric oxide (NO) may offer protection from damage during I/R. This study has looked at the functional changes and lipid alteration to mitochondria during intestinal I/R and the protection offered by NO. It was observed that I/R of the intestine is associated with functional alterations in the mitochondria as suggested by MTT reduction, respiratory control ratio and mitochondrial swelling. Mitochondrial lipid changes suggestive of activation of phospholipase A2 and phospholipase D were also seen after (I/R) mediated injury. These changes were prevented by the simultaneous presence of a NO donor in the lumen of the intestine. These studies have suggested that structural and functional alterations of mitochondria are prominent features of I/R injury to the intestine which can be ameliorated by NO. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)204-216
Number of pages13
JournalBiochimica et Biophysica Acta - Molecular Basis of Disease
Volume1500
Issue number2
DOIs
StatePublished - Feb 21 2000

Keywords

  • Intestine
  • Ischemia-reperfusion
  • Mitochondria
  • Nitric oxide
  • Phospholipase

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology

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