TY - JOUR
T1 - Attenuation of intestinal ischemia/reperfusion injury with sodium nitroprusside
T2 - Studies on mitochondrial function and lipid changes
AU - Madesh, Muniswamy
AU - Ramachandran, Anup
AU - Pulimood, Anna
AU - Vadranam, Markandeyulu
AU - Balasubramanian, K. A.
N1 - Funding Information:
The Wellcome Trust Research Laboratory is supported by The Wellcome Trust, London. Financial assistance from the Department of Science and Technology, Government of India is gratefully acknowledged. M.M. (SRF), and A.R. (SRF) are supported by the Council of Scientific and Industrial Research, India.
PY - 2000/2/21
Y1 - 2000/2/21
N2 - Reactive oxygen species have been implicated in cellular injury during ischemia/reperfusion (I/R). Mitochondria are one of the main targets of oxygen free radicals and damage to this organelle leads to cell death. Reports suggest that nitric oxide (NO) may offer protection from damage during I/R. This study has looked at the functional changes and lipid alteration to mitochondria during intestinal I/R and the protection offered by NO. It was observed that I/R of the intestine is associated with functional alterations in the mitochondria as suggested by MTT reduction, respiratory control ratio and mitochondrial swelling. Mitochondrial lipid changes suggestive of activation of phospholipase A2 and phospholipase D were also seen after (I/R) mediated injury. These changes were prevented by the simultaneous presence of a NO donor in the lumen of the intestine. These studies have suggested that structural and functional alterations of mitochondria are prominent features of I/R injury to the intestine which can be ameliorated by NO. Copyright (C) 2000 Elsevier Science B.V.
AB - Reactive oxygen species have been implicated in cellular injury during ischemia/reperfusion (I/R). Mitochondria are one of the main targets of oxygen free radicals and damage to this organelle leads to cell death. Reports suggest that nitric oxide (NO) may offer protection from damage during I/R. This study has looked at the functional changes and lipid alteration to mitochondria during intestinal I/R and the protection offered by NO. It was observed that I/R of the intestine is associated with functional alterations in the mitochondria as suggested by MTT reduction, respiratory control ratio and mitochondrial swelling. Mitochondrial lipid changes suggestive of activation of phospholipase A2 and phospholipase D were also seen after (I/R) mediated injury. These changes were prevented by the simultaneous presence of a NO donor in the lumen of the intestine. These studies have suggested that structural and functional alterations of mitochondria are prominent features of I/R injury to the intestine which can be ameliorated by NO. Copyright (C) 2000 Elsevier Science B.V.
KW - Intestine
KW - Ischemia-reperfusion
KW - Mitochondria
KW - Nitric oxide
KW - Phospholipase
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U2 - 10.1016/S0925-4439(99)00107-6
DO - 10.1016/S0925-4439(99)00107-6
M3 - Article
C2 - 10657590
AN - SCOPUS:0033958436
SN - 0925-4439
VL - 1500
SP - 204
EP - 216
JO - Biochimica et Biophysica Acta - Molecular Basis of Disease
JF - Biochimica et Biophysica Acta - Molecular Basis of Disease
IS - 2
ER -