Attenuation of cerebral vasospasm after subarachnoid hemorrhage in mice overexpressing extracellular superoxide dismutase

Matthew J. McGirt, Augusto Parra, Huaxin Sheng, Yoshinori Higuchi, Tim D. Oury, Daniel T. Laskowitz, Robert D. Pearlstein, David S. Warner

Research output: Contribution to journalArticle

80 Scopus citations

Abstract

Background and Purpose - Subarachnoid hemorrhage (SAH) increases production of vascular extracellular superoxide anion (·O2-). We examined whether overexpression of murine extracellular superoxide dismutase (EC-SOD) alters SAH-induced cerebral vasospasm, oxidative stress, and neurological outcome. Methods - Mice exhibiting a 2-fold increase in vascular EC-SOD and wild-type (WT) littermates were subjected to sham surgery or SAH by perforation of the right anterior cerebral artery. Neurological deficits were scored 72 hours later. Middle cerebral artery (MCA) diameter was measured or immunohistochemically stained for nitrotyrosine. Results - MCA diameter (mean±SD) was greater in EC-SOD versus WT mice after SAH but not sham surgery (EC-SOD SAH=56±10 μm; WT SAH=38±13 μm [P<0.01]; EC-SOD sham=99±16 μm; WT sham=100±15 μm). SAH decreased median (range) neurological score (scoring scale, 9 to 39; no deficit=39) versus shams, but there was no difference between EC-SOD and WT groups (EC-SOD SAH=26 [23 to 30]; WT SAH=23 [19 to 29] [P=0.27]; EC-SOD sham=39 [39]; WT sham=39 [39]). Sensory-motor deficits correlated with MCA diameter (P<0.001) but worsened primarily between 60 and 50 μm, plateauing below this threshold. The percentage of mice with MCA nitrotyrosine staining increased after SAH in WT (sham=29%; SAH=100% [P<0.05]) but not EC-SOD (sham=33%; SAH=44% [P=0.80]) mice. Conclusions - Endogenous overexpression of EC-SOD attenuated vasospasm and oxidative stress but failed to reduce neurological deficits after SAH. Extracellular ·O2- likely plays a direct role in the etiology of vasospasm.

Original languageEnglish (US)
Pages (from-to)2317-2323
Number of pages7
JournalStroke
Volume33
Issue number9
DOIs
StatePublished - Sep 1 2002

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Keywords

  • Extracellular space
  • Mice
  • Mice, transgenic
  • Subarachnoid hemorrhage
  • Superoxide dismutase
  • Vasospasm

ASJC Scopus subject areas

  • Clinical Neurology
  • Cardiology and Cardiovascular Medicine
  • Advanced and Specialized Nursing

Cite this

McGirt, M. J., Parra, A., Sheng, H., Higuchi, Y., Oury, T. D., Laskowitz, D. T., Pearlstein, R. D., & Warner, D. S. (2002). Attenuation of cerebral vasospasm after subarachnoid hemorrhage in mice overexpressing extracellular superoxide dismutase. Stroke, 33(9), 2317-2323. https://doi.org/10.1161/01.STR.0000027207.67639.1E