Atomic-resolution structures from fragmented protein crystals with the cryoEMEM method MicroEDED

M. Jason De La Cruz, Johan Hattne, Dan Shi, Paul Seidler, Jose Rodriguez, Francis E. Reyes, Michael R. Sawaya, Duilio Cascio, Simon C. Weiss, Sun Kyung Kim, Cynthia S. Hinck, Andrew P. Hinck, Guillermo Calero, David Eisenberg, Tamir Gonen

Research output: Contribution to journalArticlepeer-review

88 Scopus citations

Abstract

Traditionally, crystallographic analysis of macromolecules has depended on large, well-ordered crystals, which often require significant effort to obtain. Even sizable crystals sometimes suffer from pathologies that render them inappropriate for high-resolution structure determination. Here we show that fragmentation of large, imperfect crystals into microcrystals or nanocrystals can provide a simple path for high-resolution structure determination by the cryoEMEM method MicroEDED and potentially by serial femtosecond crystallography.

Original languageEnglish (US)
Pages (from-to)399-402
Number of pages4
JournalNature Methods
Volume14
Issue number4
DOIs
StatePublished - 2017
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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