Atm deficiency causes an increased frequency of intrachromosomal homologous recombination in mice

Alexander J Bishop, Carrolee Barlow, Anthony J. Wynshaw-Boris, Robert H. Schiestl

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

Ataxia telangiectasia (AT) patients have inactivating mutations in both copies of the ATM gene. The ATM protein that the gene encodes is involved in DNA double-strand break (DSB) recognition; in its absence, p53 response to DSBs is delayed and reduced. In addition, AT patients have a high propensity for cancer, and cells from these patients show chromosomal instability. Here, using an in vivo mouse model system with the pink-eyed unstable mutation, we demonstrate that the absence of functional Atm results in a significantly elevated frequency of intrachromosomal recombination resulting in deletion events (wild-type 17.73%, heterozygous Atm 15.72%, and mutant Atm 30.33%). No such increase was observed in mice heterozygous for Atm. These results further advocate the role of ATM in maintaining genomic integrity after the onset of endogenous damage. This system relies on the initiation of events during a relatively short time frame to produce an observable deletion product. AT patients have a lifelong exposure to endogenous damage and perhaps similarly acting external agents. Because 25% of our genome consists of repeated elements, genomic instability due to an increased level of homologous recombination between such repeats, as observed here, may contribute to carcinogenesis in AT patients.

Original languageEnglish (US)
Pages (from-to)395-399
Number of pages5
JournalCancer Research
Volume60
Issue number2
StatePublished - Jan 15 2000
Externally publishedYes

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Homologous Recombination
Ataxia Telangiectasia
Ataxia Telangiectasia Mutated Proteins
Chromosomal Instability
Mutation
Double-Stranded DNA Breaks
Genomic Instability
Genetic Recombination
Genes
Carcinogenesis
Genome
Neoplasms

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Atm deficiency causes an increased frequency of intrachromosomal homologous recombination in mice. / Bishop, Alexander J; Barlow, Carrolee; Wynshaw-Boris, Anthony J.; Schiestl, Robert H.

In: Cancer Research, Vol. 60, No. 2, 15.01.2000, p. 395-399.

Research output: Contribution to journalArticle

Bishop, AJ, Barlow, C, Wynshaw-Boris, AJ & Schiestl, RH 2000, 'Atm deficiency causes an increased frequency of intrachromosomal homologous recombination in mice', Cancer Research, vol. 60, no. 2, pp. 395-399.
Bishop, Alexander J ; Barlow, Carrolee ; Wynshaw-Boris, Anthony J. ; Schiestl, Robert H. / Atm deficiency causes an increased frequency of intrachromosomal homologous recombination in mice. In: Cancer Research. 2000 ; Vol. 60, No. 2. pp. 395-399.
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