Association between HIV-1 tropism and CCR5 human haplotype E in a Caucasian population

Kristi Huik, Radko Avi, Helen Uibopuu, Merit Pauskar, Tõnu Margus, Tõnis Karki, Tõnu Krispin, Piret Kool, Kristi Rüütel, Ave Talu, Katri Abel-Ollo, Anneli Uusküla, Andrew Carrillo, Weijing He, Sunil K. Ahuja, Irja Lutsar

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Background: The influence of the diversity of CCR5 on HIV susceptibility and disease progression has been clearly demonstrated but how the variability of this gene influences the HIV tropism is poorly understood. We investigated whether CCR5 haplotypes are associated with HIV tropism in a Caucasian population. Methods: We evaluated 161 HIV-positive subjects in a crosssectional study. CCR5 haplotypes were derived after genotyping 9 CCR2-CCR5 polymorphisms. The HIV subtype was determined by phylogenetic analysis using the maximum likelihood method and viral tropism by the genotypic tropism assay (geno2pheno). Associations between CCR5 haplotypes and viral tropism were determined using logistic regression analyses. Samples from 500 blood donors were used to evaluate the representativeness of HIV-positives in terms of CCR5 haplotype distribution. Results: The distribution of CCR5 haplotypes was similar in HIVpositive subjects and blood donors. The majority of viruses (93.8%) belonged to HIV-1 CRF06-cpx; 7.5% were X4, and the remaining were R5 tropic. X4 tropic viruses were over represented among people with CCR5 human haplotype E (HHE) compared with those without this haplotype (13.0% vs 1.4%; P = 0.006). People possessing CCR5 HHE had 11 times increased odds (odds ratio = 11.00; 95% confidence interval: 1.38 to 87.38) of having X4 tropic viruses than those with non-HHE. After adjusting for antiretroviral (ARV) therapy, neither the presence of HHE nor the use of ARV was associated with X4 tropic viruses. Conclusions: Our results suggest that CCR5 HHE and ARV treatment might be associated with the presence of HIV-1 X4 tropic viruses.

Original languageEnglish (US)
Pages (from-to)239-244
Number of pages6
JournalJournal of Acquired Immune Deficiency Syndromes
Volume66
Issue number3
DOIs
StatePublished - Jul 1 2014

Keywords

  • CCR5
  • Intravenous drug users
  • X4 tropic viruses

ASJC Scopus subject areas

  • Infectious Diseases
  • Pharmacology (medical)

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    Huik, K., Avi, R., Uibopuu, H., Pauskar, M., Margus, T., Karki, T., Krispin, T., Kool, P., Rüütel, K., Talu, A., Abel-Ollo, K., Uusküla, A., Carrillo, A., He, W., Ahuja, S. K., & Lutsar, I. (2014). Association between HIV-1 tropism and CCR5 human haplotype E in a Caucasian population. Journal of Acquired Immune Deficiency Syndromes, 66(3), 239-244. https://doi.org/10.1097/QAI.0000000000000127