Association between acetylator genotype and 2-amino-1-methyl-6- phenylimidazo[4,5-b]pyridine (PhIP) DNA adduct formation in colon and prostate of inbred Fischer 344 and Wistar Kyoto rats

Madhu Purewal, Adrian J. Fretland, Herman A.J. Schut, David W. Hein, Michael J. Wargovich

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP), a heterocyclic amine (HCA) found in cooked meats, causes colon and prostate tumors in male rats. Polymorphic N-acetyltransferase metabolizes N-hydroxy-PhIP to a DNA- reactive form. Liver, colon, and prostate PhIP-DNA adduct levels were compared in male rapid-acetylator Fischer 344 (F344) and slow-acetylator Wistar-Kyoto (WKY) rats fed 0.01 or 0.04% PhIP. Liver PhIP-DNA adduct levels at both PhIP doses, and colon PhIP-DNA adduct levels at the 0.01% PhIP dose were unaffected by acetylator genotype. However, in rats fed 0.04% PhIP, colon PhIP-DNA adduct levels were higher in rapid acetylator F344 rats (P<0.05). Similarly, prostate PhIP-DNA adduct levels were higher in rapid acetylator F344 rats at both PhIP doses (P<0.05). The combination of the high-PhIP dose and rapid-acetylator genotype resulted in the highest level of PhIP-DNA adducts in rat colon and prostate. (C) 2000 Elsevier Science Ireland Ltd.

Original languageEnglish (US)
Pages (from-to)53-60
Number of pages8
JournalCancer Letters
Volume149
Issue number1-2
DOIs
StatePublished - Feb 28 2000
Externally publishedYes

Keywords

  • 2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP)
  • DNA adducts
  • Heterocyclic amines
  • Inbred rats
  • N-Acetyltransferase

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Fingerprint Dive into the research topics of 'Association between acetylator genotype and 2-amino-1-methyl-6- phenylimidazo[4,5-b]pyridine (PhIP) DNA adduct formation in colon and prostate of inbred Fischer 344 and Wistar Kyoto rats'. Together they form a unique fingerprint.

  • Cite this