Assignment of Xeroderma Pigmentosum Group C (XPC) Gene to Chromosome 3p25

Randy J. Legerski, Pu Liu, Lei Li, Carolyn A. Peterson, Ying Zhao, Robin J Leach, Susan L. Naylor, Michael J. Siciliano

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The human gene XPC (formerly designated XPCC), which corrects the repair deficiency of xeroderma pigmentosum (XP) group C cells, was mapped to 3p25. A cDNA probe for Southern blot hybridization and diagnostic PCR analyses of hybrid clone panels informative for human chromosomes in general and portions of chromosome 3 in particular produced the initial results. Fluorescence in situ hybridization utilizing both a yeast artificial chromosome DNA containing the gene and XPC cDNA as probes provided verification and specific regional assignment. A conflicting assignment of XPC to chromosome 5 is discussed in light of inadequacies in the exclusive use of microcell-mediated chromosome transfer for gene mapping.

Original languageEnglish (US)
Pages (from-to)266-269
Number of pages4
JournalGenomics
Volume21
Issue number1
DOIs
StatePublished - May 1 1994

Fingerprint

Xeroderma Pigmentosum
Chromosomes
Genes
Complementary DNA
Chromosomes, Human, 6-12 and X
Yeast Artificial Chromosomes
Chromosomes, Human, Pair 5
Chromosomes, Human, Pair 3
Chromosome Mapping
Human Chromosomes
Southern Blotting
Fluorescence In Situ Hybridization
Clone Cells
Polymerase Chain Reaction
DNA

ASJC Scopus subject areas

  • Genetics

Cite this

Legerski, R. J., Liu, P., Li, L., Peterson, C. A., Zhao, Y., Leach, R. J., ... Siciliano, M. J. (1994). Assignment of Xeroderma Pigmentosum Group C (XPC) Gene to Chromosome 3p25. Genomics, 21(1), 266-269. https://doi.org/10.1006/geno.1994.1256

Assignment of Xeroderma Pigmentosum Group C (XPC) Gene to Chromosome 3p25. / Legerski, Randy J.; Liu, Pu; Li, Lei; Peterson, Carolyn A.; Zhao, Ying; Leach, Robin J; Naylor, Susan L.; Siciliano, Michael J.

In: Genomics, Vol. 21, No. 1, 01.05.1994, p. 266-269.

Research output: Contribution to journalArticle

Legerski, RJ, Liu, P, Li, L, Peterson, CA, Zhao, Y, Leach, RJ, Naylor, SL & Siciliano, MJ 1994, 'Assignment of Xeroderma Pigmentosum Group C (XPC) Gene to Chromosome 3p25', Genomics, vol. 21, no. 1, pp. 266-269. https://doi.org/10.1006/geno.1994.1256
Legerski, Randy J. ; Liu, Pu ; Li, Lei ; Peterson, Carolyn A. ; Zhao, Ying ; Leach, Robin J ; Naylor, Susan L. ; Siciliano, Michael J. / Assignment of Xeroderma Pigmentosum Group C (XPC) Gene to Chromosome 3p25. In: Genomics. 1994 ; Vol. 21, No. 1. pp. 266-269.
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