Assignment of the human peroxisomal branched-chain Acyl-CoA oxidase gene to chromosome 3p21.1-p14.2 by rodent/human somatic cell hybridization

Nabil N. Moghrabi, Susan L. Naylor, Paul P. Van Veldhoven, Eveline Baumgart, D. Brian Dawson, Michael J. Bennett

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

PCR and rodent/human somatic cell hybrids were used to localize the human peroxisomal branched chain acyl-CoA oxidase gene. Oligonucleotide primers were chosen to specifically amplify human hBCox DNA. The amplified sequence contained two restriction enzyme sites which were used to verify the authenticity of the amplified DNA. Initially, the gene was localized to human chromosome 3 by screening genomic DNA from a hybrid mapping panel. Additional hybrids retaining well-characterized fragments of human chromosome 3 were screened to further localize the gene to 3p21.1-p14.2.

Original languageEnglish (US)
Pages (from-to)767-769
Number of pages3
JournalBiochemical and Biophysical Research Communications
Volume231
Issue number3
DOIs
StatePublished - Feb 24 1997

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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