Assessment of molecular typing methods to determine invasiveness and to differentiate clones of Streptococcus pneumoniae

Caroline A. Obert, Geli Gao, Jack Sublett, Elaine I. Tuomanen, Carlos J. Orihuela

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

In the United States, Streptococcus pneumoniae is the leading cause of community-acquired pneumonia and invasive bacterial disease. As antimicrobial resistance increases, it will become critical to determine if strains circulating in a population are likely to cause invasive pneumococcal disease (IPD). This is possible by comparison of an isolate's genotype to strains known to be invasive. In this work, we compared pulse-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST), comparative genomic hybridization (CGH) and multi-invasive-locus sequence typing (MILST) for their ability to distinguish between known IPD causing and carrier strains using phylogenetic analyses. In addition, we assess the ability of these techniques to determine true clones from highly related strains. The resulting trees suggest that despite similar overall topologies, the clearest picture of invasiveness and genetic relatedness can be viewed when typing methods are used collectively.

Original languageEnglish (US)
Pages (from-to)708-716
Number of pages9
JournalInfection, Genetics and Evolution
Volume7
Issue number6
DOIs
StatePublished - Dec 2007

Keywords

  • CGH
  • Comparison
  • MILST
  • MLST
  • PFGE
  • Phylogenetics
  • Pneumococcus

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics
  • Microbiology (medical)
  • Infectious Diseases

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