TY - JOUR
T1 - Assessment of genetic damage in peripheral blood of human volunteers exposed (whole-body) to a 200 T, 60Hz magnetic field
AU - Albert, Genevieve C.
AU - McNamee, James P.
AU - Marro, Leonora
AU - Vijayalaxmi,
AU - Bellier, Pascale V.
AU - Prato, Frank S.
AU - Thomas, Alex W.
N1 - Funding Information:
The authors are grateful to Canadian Institute of Health Research (CIHR) and the Natural Sciences Engineering Research Council (NSERC) for funding this investigation. We thank Dr Scott Karnas and the personnel in the laboratories of Dr Jeff Carson and Dr Alexandre Alegros at the Lawson Health Research Institute, and the staff at the Department of Nuclear Medicine, St Joseph’s Health Care hospital for their help throughout this study.
PY - 2009/2
Y1 - 2009/2
N2 - Aim: To investigate the extent of damage in nucleated cells in peripheral blood of healthy human volunteers exposed to a whole-body 60Hz, 200T magnetic field. Materials and methods: In this study, 10 male and 10 female healthy human volunteers received a 4h whole-body exposure to a 200T, 60Hz magnetic field. In addition, five males and five females were treated in a similar fashion, but were exposed to sham conditions. For each subject, a blood sample was obtained prior to the exposure period and aliquots were used as negative- (pre-exposure) and positive- [1.5 Gray (Gy) 60Cobalt (60Co) -irradiation] controls. At the end of the 4h exposure period, a second blood sample was obtained. The extent of DNA damage was assessed in peripheral human blood leukocytes from all samples using the alkaline comet assay. To detect possible clastogenic effects, the incidence of micronuclei was assessed in phytohemagglutinin (PHA)-stimulated lymphocytes using the cytokinesis-block micronucleus assay. Results: There was no evidence of either increased DNA damage, as indicated by the alkaline comet assay, or increased incidence of micronuclei (MN) in the magnetic field exposed group. However, an invitro exposure of 1.5Gy -irradiation caused a significant increase in both DNA damage and MN induction. Conclusions: This study found no evidence that an acute, whole-body exposure to a 200T, 60Hz magnetic field for 4 hours could cause DNA damage in human blood.
AB - Aim: To investigate the extent of damage in nucleated cells in peripheral blood of healthy human volunteers exposed to a whole-body 60Hz, 200T magnetic field. Materials and methods: In this study, 10 male and 10 female healthy human volunteers received a 4h whole-body exposure to a 200T, 60Hz magnetic field. In addition, five males and five females were treated in a similar fashion, but were exposed to sham conditions. For each subject, a blood sample was obtained prior to the exposure period and aliquots were used as negative- (pre-exposure) and positive- [1.5 Gray (Gy) 60Cobalt (60Co) -irradiation] controls. At the end of the 4h exposure period, a second blood sample was obtained. The extent of DNA damage was assessed in peripheral human blood leukocytes from all samples using the alkaline comet assay. To detect possible clastogenic effects, the incidence of micronuclei was assessed in phytohemagglutinin (PHA)-stimulated lymphocytes using the cytokinesis-block micronucleus assay. Results: There was no evidence of either increased DNA damage, as indicated by the alkaline comet assay, or increased incidence of micronuclei (MN) in the magnetic field exposed group. However, an invitro exposure of 1.5Gy -irradiation caused a significant increase in both DNA damage and MN induction. Conclusions: This study found no evidence that an acute, whole-body exposure to a 200T, 60Hz magnetic field for 4 hours could cause DNA damage in human blood.
KW - DNA damage
KW - Magnetic field
KW - Micronuclei
KW - Non-ionizing radiation
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U2 - 10.1080/09553000802641169
DO - 10.1080/09553000802641169
M3 - Article
C2 - 19280467
AN - SCOPUS:65649095211
VL - 85
SP - 144
EP - 152
JO - International Journal of Radiation Biology
JF - International Journal of Radiation Biology
SN - 0955-3002
IS - 2
ER -