Assessment of airway hyperresponsiveness in murine tracheal rings

Jeremiah T. Herlihy, Iurii Semenov, Robert Brenner

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Isolated tracheal rings have often been used to directly measure the contractile output of airway smooth muscle (ASM). Here, we describe the method for excising murine tracheas, mounting tracheal rings in organ baths, and measuring the isometric forces generated by the ASM when stimulated by drug additions or electric field stimulation. The apparatus for the setup and the pathways responsible for stimulation are detailed. Examples of the responses and analyses of two types of ASM stimulation are illustrated: (1) the carbachol concentration-response curve and (2) the frequency-response curve elicited by electric field stimulation.

Original languageEnglish (US)
Title of host publicationMouse Models of Allergic Disease
Subtitle of host publicationMethods and Protocols
PublisherHumana Press
Pages257-269
Number of pages13
ISBN (Print)9781627034951
DOIs
StatePublished - 2013

Publication series

NameMethods in Molecular Biology
Volume1032
ISSN (Print)1064-3745

Keywords

  • Airway smooth muscle
  • Concentration-response curves
  • Electric field stimulation
  • Hyperresponsiveness
  • Isometric force
  • Tracheal rings

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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