Assembly of the base excision repair complex on abasic DNA and role of adenomatous polyposis coli on its functional activity

Aruna S. Jaiswal, Satya Narayan

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

The assembly and stability of base excision repair (BER) proteins in vivo with abasic DNA and the role of adenomatous polyposis coli (APC) protein in this process are currently unclear. We have studied the assembly of a multiprotein BER complex onto abasic DNA (F-DNA) and characterized the physical and functional activity of the associated proteins. We found that the BER complex contained all the essential components of the long-patch BER system, such as APE1, Pol-β, Fen1, and DNA ligase I. Interestingly, wild-type APC was also present in the BER complex. Kinetics of the assembly of BER proteins onto the F-DNA were rapid and appeared in sequential order depending upon their requirement in the repair process. The presence of wild-type APC in the BER complex caused a decrease in the level of assembly of BER proteins and negatively affected long-patch BER. These results suggest that major BER proteins in the complex are assembled onto F-DNA and are competent in performing DNA repair. Wild-type APC in the BER complex reduces the repair activity, probably because of interaction with multiple components of the system.

Original languageEnglish (US)
Pages (from-to)1901-1909
Number of pages9
JournalBiochemistry
Volume50
Issue number11
DOIs
StatePublished - Mar 22 2011
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry

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