Assay of β-glucuronidase in bile following ion-pair extraction of pigments and bile acids

William E. Kurtin, Wayne H. Schwesinger

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

A method for improving the assay of β-glucuronidase in hepatic and gallbladder bile is described. The method uses ion-pair extraction with N,N,N-triheptyl-l-heptanaminium bromide to remove pigments and bile acids. Conjugated bilirubin, unconjugated bilirubin, and taurine and glycine conjugates of deoxycholic and chenodeoxycholic acids are extracted efficiently from bile by the procedure. The sensitivity of the spectrophotometric assay of β-glucuronidase in bile using phenolphthalein glucuronide is increased significantly.

Original languageEnglish (US)
Pages (from-to)511-516
Number of pages6
JournalAnalytical Biochemistry
Volume147
Issue number2
DOIs
StatePublished - Jun 1985

Keywords

  • bile acids
  • bile analysis
  • detergents (surfactants)
  • hydrolases
  • ion-pair extraction
  • pigments
  • β-glucuronidase

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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