Assay of β-glucuronidase in bile following ion-pair extraction of pigments and bile acids

William E. Kurtin; Wayne H. Schwesinger

doi:10.1016/0003-2697(85)90306-9

Assay of β-glucuronidase in bile following ion-pair extraction of pigments and bile acids

William E. Kurtin, Wayne H. Schwesinger

Department of Surgery

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

A method for improving the assay of β-glucuronidase in hepatic and gallbladder bile is described. The method uses ion-pair extraction with N,N,N-triheptyl-l-heptanaminium bromide to remove pigments and bile acids. Conjugated bilirubin, unconjugated bilirubin, and taurine and glycine conjugates of deoxycholic and chenodeoxycholic acids are extracted efficiently from bile by the procedure. The sensitivity of the spectrophotometric assay of β-glucuronidase in bile using phenolphthalein glucuronide is increased significantly.

Original language	English (US)
Pages (from-to)	511-516
Number of pages	6
Journal	Analytical Biochemistry
Volume	147
Issue number	2
DOIs	https://doi.org/10.1016/0003-2697(85)90306-9
State	Published - Jun 1985

Keywords

bile acids
bile analysis
detergents (surfactants)
hydrolases
ion-pair extraction
pigments
β-glucuronidase

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1016/0003-2697(85)90306-9

Cite this

@article{71bf7ef1ca6b4bf3a0bc51480dc95695,

title = "Assay of β-glucuronidase in bile following ion-pair extraction of pigments and bile acids",

abstract = "A method for improving the assay of β-glucuronidase in hepatic and gallbladder bile is described. The method uses ion-pair extraction with N,N,N-triheptyl-l-heptanaminium bromide to remove pigments and bile acids. Conjugated bilirubin, unconjugated bilirubin, and taurine and glycine conjugates of deoxycholic and chenodeoxycholic acids are extracted efficiently from bile by the procedure. The sensitivity of the spectrophotometric assay of β-glucuronidase in bile using phenolphthalein glucuronide is increased significantly.",

keywords = "bile acids, bile analysis, detergents (surfactants), hydrolases, ion-pair extraction, pigments, β-glucuronidase",

author = "Kurtin, {William E.} and Schwesinger, {Wayne H.}",

year = "1985",

month = jun,

doi = "10.1016/0003-2697(85)90306-9",

language = "English (US)",

volume = "147",

pages = "511--516",

journal = "Analytical Biochemistry",

issn = "0003-2697",

publisher = "Academic Press Inc.",

number = "2",

}

TY - JOUR

T1 - Assay of β-glucuronidase in bile following ion-pair extraction of pigments and bile acids

AU - Kurtin, William E.

AU - Schwesinger, Wayne H.

PY - 1985/6

Y1 - 1985/6

N2 - A method for improving the assay of β-glucuronidase in hepatic and gallbladder bile is described. The method uses ion-pair extraction with N,N,N-triheptyl-l-heptanaminium bromide to remove pigments and bile acids. Conjugated bilirubin, unconjugated bilirubin, and taurine and glycine conjugates of deoxycholic and chenodeoxycholic acids are extracted efficiently from bile by the procedure. The sensitivity of the spectrophotometric assay of β-glucuronidase in bile using phenolphthalein glucuronide is increased significantly.

AB - A method for improving the assay of β-glucuronidase in hepatic and gallbladder bile is described. The method uses ion-pair extraction with N,N,N-triheptyl-l-heptanaminium bromide to remove pigments and bile acids. Conjugated bilirubin, unconjugated bilirubin, and taurine and glycine conjugates of deoxycholic and chenodeoxycholic acids are extracted efficiently from bile by the procedure. The sensitivity of the spectrophotometric assay of β-glucuronidase in bile using phenolphthalein glucuronide is increased significantly.

KW - bile acids

KW - bile analysis

KW - detergents (surfactants)

KW - hydrolases

KW - ion-pair extraction

KW - pigments

KW - β-glucuronidase

UR - http://www.scopus.com/inward/record.url?scp=0021794013&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021794013&partnerID=8YFLogxK

U2 - 10.1016/0003-2697(85)90306-9

DO - 10.1016/0003-2697(85)90306-9

M3 - Article

C2 - 4014691

AN - SCOPUS:0021794013

SN - 0003-2697

VL - 147

SP - 511

EP - 516

JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 2

ER -

Assay of β-glucuronidase in bile following ion-pair extraction of pigments and bile acids

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this