Assay for assessing mitochondrial function in iPSC-derived neural stem cells and dopaminergic neurons

Gourav Roy-Choudhury, Marcel M. Daadi

Research output: Chapter in Book/Report/Conference proceedingChapter

6 Scopus citations

Abstract

Rapid and reliable assessment of mitochondrial bioenergetics is a vital tool in drug discovery studies aimed at reversing or improving mitochondrial dysfunction. Induced pluripotent stem cell (iPSC)-derived neural stem cells (NSCs) carry and replicate the donor disease pathology and can be an ideal cellular model for phenotypic screening of compounds. Herein we describe the use of Seahorse XFe96 analyzer to assess mitochondrial functions in iPSC-derived NSCs for drug screening.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press
Pages161-173
Number of pages13
DOIs
StatePublished - 2019

Publication series

NameMethods in Molecular Biology
Volume1919
ISSN (Print)1064-3745

Keywords

  • Drug screening
  • Kinase inhibitors
  • LRRK-2
  • Mitochondrial functions
  • Parkinson’s disease
  • iPSCs

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Fingerprint

Dive into the research topics of 'Assay for assessing mitochondrial function in iPSC-derived neural stem cells and dopaminergic neurons'. Together they form a unique fingerprint.

Cite this