Ascorbic acid stimulates the resorption of canine articular cartilage induced by a factor derived from activated rabbit macrophages

D. D. Dean, A. Sellers, D. S. Howell, S. S. Kerwar, J. F. Woessner

Research output: Contribution to journalArticlepeer-review

Abstract

Articular cartilage explants from the knees of mongrel dogs release 5-10% of their proteoglycan content spontaneously when cultured for 4 days in serum-free modified Bigger's medium. A factor synthesized and secreted by lipopolysaccharide-stimulated rabbit macrophages can stimulate this release of proteoglycan by 2 to 3-fold. The release of proteoglycan in response to macrophage factor is maximal in the presence of 1.5-50 μg/ml l-ascorbic acid. In the absence of ascorbate, or with high levels of ascorbate (150 μg/ml), the effect of the factor is diminished by 50%. d-isoascorbate, reduced glutathione, or dithiothreitol cannot substitute for l-ascorbate in producing this effect, while dehydroascorbate can.

Original languageEnglish (US)
Pages (from-to)103-107
Number of pages5
JournalRheumatology International
Volume5
Issue number3
DOIs
StatePublished - May 1 1985

Keywords

  • Ascorbic acid
  • Cartilage resorption
  • Macrophage-derived factor

ASJC Scopus subject areas

  • Rheumatology
  • Immunology and Allergy
  • Immunology

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