Arterial thrombosis induces early upregulation of intercellular adhesion molecule in the media

B. Toursarkissian, D. Schwartz, P. R. Eisenberg, B. G. Rubin

Research output: Contribution to journalArticle

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Abstract

Purpose: Thrombosed peripheral vessels that are pharmacologically or mechanically recanalized have diminished long-term patency rates compared with vessels that are repaired before occlusion. We hypothesized that thrombosis induces proinflammatory changes in the arterial media that may contribute to postthrombotic vascular remodeling. Methods: We studied expression of intercellular adhesion molecule (ICAM), a mediator of leukocyte recruitment, in the arterial wall after thrombosis. Thrombosis was induced in rabbit superficial femoral arteries by embolizing polystyrene beads (Thr- emb) or by ligation (Thr-lig). Control vessels were dissected but not ligated (C-dis) or were subjected to bead embolization and immediate removal (C- emb). Arterial wall ICAM expression was measured by indirect immunohistochemical analysis at 6 hours, 24 hours, and 1 week. Staining intensity was graded from 0 (none) to 4 (intense) by observers who were blinded to the experimental conditions. Results: No increase in ICAM expression by thrombosed vessels was present at 6 hours. After 24 hours, ICAM expression in the media of thrombosed vessels was increased (Thr-emb, 2.3 ± 0.5; Thr-lig, 2.0 ± 0) compared with control vessels (C-dis, 0 ± 0; C-emb, 0.8 ± 0.5; p < 0.004). This difference became more marked at 1 week. ICAM staining localized to actin-staining regions of the media. Conclusions: Arterial thrombosis, but not surgical injury, induces pronounced early and sustained upregulation of ICAM expression in smooth muscle-containing regions of the arterial media. Upregulation of ICAM is likely to promote recruitment of inflammatory cells or mediate vascular remodeling after luminal thrombosis.

Original languageEnglish (US)
Pages (from-to)663-669
Number of pages7
JournalJournal of Vascular Surgery
Volume26
Issue number4
DOIs
StatePublished - 1997
Externally publishedYes

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Cell Adhesion Molecules
Thrombosis
Up-Regulation
Tunica Media
Staining and Labeling
Polystyrenes
Intraoperative Complications
Femoral Artery
Ligation
Smooth Muscle
Actins
Leukocytes
Rabbits

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Surgery

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Arterial thrombosis induces early upregulation of intercellular adhesion molecule in the media. / Toursarkissian, B.; Schwartz, D.; Eisenberg, P. R.; Rubin, B. G.

In: Journal of Vascular Surgery, Vol. 26, No. 4, 1997, p. 663-669.

Research output: Contribution to journalArticle

Toursarkissian, B. ; Schwartz, D. ; Eisenberg, P. R. ; Rubin, B. G. / Arterial thrombosis induces early upregulation of intercellular adhesion molecule in the media. In: Journal of Vascular Surgery. 1997 ; Vol. 26, No. 4. pp. 663-669.
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