Approaches to cloning of pain-related ion channel genes

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations

Abstract

Molecular pain research is a relatively new and rapidly expanding field that represents advancement in conventional pain research. One of the fundamentals of molecular pain involves the cloning of genes and especially the ion channels specifically involved in nociceptive processing at the periphery and centrally. A variety of approaches were used to isolate these critically important genes. Cloning of these genes involved innovative strategies based on existing molecular approaches. This review will discuss well-utilized cloning approaches and their exploitation in molecular pain research.

Original languageEnglish (US)
Title of host publicationIon Channels
Subtitle of host publicationMethods and Protocols
EditorsNikita Gamper
Pages3-19
Number of pages17
DOIs
StatePublished - 2013

Publication series

NameMethods in Molecular Biology
Volume998
ISSN (Print)1064-3745

Keywords

  • Differential cloning
  • Expression cloning
  • Functional cloning
  • Homology cloning
  • Molecular pain research
  • Subtractive cloning

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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