Abstract
Molecular pain research is a relatively new and rapidly expanding field that represents advancement in conventional pain research. One of the fundamentals of molecular pain involves the cloning of genes and especially the ion channels specifically involved in nociceptive processing at the periphery and centrally. A variety of approaches were used to isolate these critically important genes. Cloning of these genes involved innovative strategies based on existing molecular approaches. This review will discuss well-utilized cloning approaches and their exploitation in molecular pain research.
| Original language | English (US) |
|---|---|
| Title of host publication | Methods in Molecular Biology |
| Pages | 3-19 |
| Number of pages | 17 |
| Volume | 998 |
| DOIs | |
| State | Published - 2013 |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 998 |
| ISSN (Print) | 10643745 |
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Keywords
- Differential cloning
- Expression cloning
- Functional cloning
- Homology cloning
- Molecular pain research
- Subtractive cloning
ASJC Scopus subject areas
- Molecular Biology
- Genetics
Cite this
Approaches to cloning of pain-related ion channel genes. / Akopian, Armen N.
Methods in Molecular Biology. Vol. 998 2013. p. 3-19 (Methods in Molecular Biology; Vol. 998).Research output: Chapter in Book/Report/Conference proceeding › Chapter
}
TY - CHAP
T1 - Approaches to cloning of pain-related ion channel genes
AU - Akopian, Armen N
PY - 2013
Y1 - 2013
N2 - Molecular pain research is a relatively new and rapidly expanding field that represents advancement in conventional pain research. One of the fundamentals of molecular pain involves the cloning of genes and especially the ion channels specifically involved in nociceptive processing at the periphery and centrally. A variety of approaches were used to isolate these critically important genes. Cloning of these genes involved innovative strategies based on existing molecular approaches. This review will discuss well-utilized cloning approaches and their exploitation in molecular pain research.
AB - Molecular pain research is a relatively new and rapidly expanding field that represents advancement in conventional pain research. One of the fundamentals of molecular pain involves the cloning of genes and especially the ion channels specifically involved in nociceptive processing at the periphery and centrally. A variety of approaches were used to isolate these critically important genes. Cloning of these genes involved innovative strategies based on existing molecular approaches. This review will discuss well-utilized cloning approaches and their exploitation in molecular pain research.
KW - Differential cloning
KW - Expression cloning
KW - Functional cloning
KW - Homology cloning
KW - Molecular pain research
KW - Subtractive cloning
UR - http://www.scopus.com/inward/record.url?scp=84877086382&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84877086382&partnerID=8YFLogxK
U2 - 10.1007/978-1-62703-351-0_1
DO - 10.1007/978-1-62703-351-0_1
M3 - Chapter
C2 - 23529417
AN - SCOPUS:84877086382
SN - 9781627033503
VL - 998
T3 - Methods in Molecular Biology
SP - 3
EP - 19
BT - Methods in Molecular Biology
ER -