Applications of PLOR in labeling large RNAs at specific sites

Yu Liu, Ping Yu, Marzena Dyba, Rui J Sousa, Jason R. Stagno, Yun Xing Wang

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Incorporation of modified or labeled nucleotides at specific sites in RNAs is critical for gaining insights into the structure and function of RNAs. Preparation of site-specifically labeled large RNAs in amounts suitable for structural or functional studies is extremely difficult using current methodologies. The position-selective labeling of RNA, PLOR, is a recently developed method that makes such syntheses possible. PLOR allows incorporation of various probes, including 2D/13C/15N-isotopic labels, Cy3/Cy5/Alexa488/Alexa555 fluorescent dyes, biotin and other chemical groups, into specific positions in long RNAs. Here, we describe in detail the use of PLOR to label RNAs at specific segment(s) or discrete sites.

Original languageEnglish (US)
JournalMethods
DOIs
StateAccepted/In press - Feb 2 2016

Keywords

  • PLOR
  • RNA
  • Specific labeling

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)

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