Applications of PLOR in labeling large RNAs at specific sites

Yu Liu; Ping Yu; Marzena Dyba; Rui Sousa; Jason R. Stagno; Yun Xing Wang

doi:10.1016/j.ymeth.2016.03.014

Applications of PLOR in labeling large RNAs at specific sites

Yu Liu, Ping Yu, Marzena Dyba, Rui Sousa, Jason R. Stagno, Yun Xing Wang

Biochemistry & Structural Biology

Research output: Contribution to journal › Review article › peer-review

14 Scopus citations

Abstract

Incorporation of modified or labeled nucleotides at specific sites in RNAs is critical for gaining insights into the structure and function of RNAs. Preparation of site-specifically labeled large RNAs in amounts suitable for structural or functional studies is extremely difficult using current methodologies. The position-selective labeling of RNA, PLOR, is a recently developed method that makes such syntheses possible. PLOR allows incorporation of various probes, including ²D/¹³C/¹⁵N-isotopic labels, Cy3/Cy5/Alexa488/Alexa555 fluorescent dyes, biotin and other chemical groups, into specific positions in long RNAs. Here, we describe in detail the use of PLOR to label RNAs at specific segment(s) or discrete sites.

Original language	English (US)
Pages (from-to)	4-10
Number of pages	7
Journal	Methods
Volume	103
DOIs	https://doi.org/10.1016/j.ymeth.2016.03.014
State	Published - Jul 1 2016

Keywords

PLOR
RNA
Specific labeling

ASJC Scopus subject areas

Molecular Biology
Biochemistry, Genetics and Molecular Biology(all)

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10.1016/j.ymeth.2016.03.014

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title = "Applications of PLOR in labeling large RNAs at specific sites",

abstract = "Incorporation of modified or labeled nucleotides at specific sites in RNAs is critical for gaining insights into the structure and function of RNAs. Preparation of site-specifically labeled large RNAs in amounts suitable for structural or functional studies is extremely difficult using current methodologies. The position-selective labeling of RNA, PLOR, is a recently developed method that makes such syntheses possible. PLOR allows incorporation of various probes, including 2D/13C/15N-isotopic labels, Cy3/Cy5/Alexa488/Alexa555 fluorescent dyes, biotin and other chemical groups, into specific positions in long RNAs. Here, we describe in detail the use of PLOR to label RNAs at specific segment(s) or discrete sites.",

keywords = "PLOR, RNA, Specific labeling",

author = "Yu Liu and Ping Yu and Marzena Dyba and Rui Sousa and Stagno, {Jason R.} and Wang, {Yun Xing}",

note = "Funding Information: This work was supported by the Intramural Research Programs of the National Cancer Institute. The content of this publication does not necessarily reflect the views or policies of the Department of Health and Human Services, nor does mention of trade names, commercial products, or organizations imply endorsement by the US government. Publisher Copyright: {\textcopyright} 2016.",

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AU - Yu, Ping

AU - Dyba, Marzena

AU - Sousa, Rui

AU - Stagno, Jason R.

AU - Wang, Yun Xing

N1 - Funding Information: This work was supported by the Intramural Research Programs of the National Cancer Institute. The content of this publication does not necessarily reflect the views or policies of the Department of Health and Human Services, nor does mention of trade names, commercial products, or organizations imply endorsement by the US government. Publisher Copyright: © 2016.

PY - 2016/7/1

Y1 - 2016/7/1

N2 - Incorporation of modified or labeled nucleotides at specific sites in RNAs is critical for gaining insights into the structure and function of RNAs. Preparation of site-specifically labeled large RNAs in amounts suitable for structural or functional studies is extremely difficult using current methodologies. The position-selective labeling of RNA, PLOR, is a recently developed method that makes such syntheses possible. PLOR allows incorporation of various probes, including 2D/13C/15N-isotopic labels, Cy3/Cy5/Alexa488/Alexa555 fluorescent dyes, biotin and other chemical groups, into specific positions in long RNAs. Here, we describe in detail the use of PLOR to label RNAs at specific segment(s) or discrete sites.

AB - Incorporation of modified or labeled nucleotides at specific sites in RNAs is critical for gaining insights into the structure and function of RNAs. Preparation of site-specifically labeled large RNAs in amounts suitable for structural or functional studies is extremely difficult using current methodologies. The position-selective labeling of RNA, PLOR, is a recently developed method that makes such syntheses possible. PLOR allows incorporation of various probes, including 2D/13C/15N-isotopic labels, Cy3/Cy5/Alexa488/Alexa555 fluorescent dyes, biotin and other chemical groups, into specific positions in long RNAs. Here, we describe in detail the use of PLOR to label RNAs at specific segment(s) or discrete sites.

KW - PLOR

KW - RNA

KW - Specific labeling

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DO - 10.1016/j.ymeth.2016.03.014

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SP - 4

EP - 10

JO - Methods

JF - Methods

SN - 1046-2023

ER -

Applications of PLOR in labeling large RNAs at specific sites

Abstract

Keywords

ASJC Scopus subject areas

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