Applications of CpG island microarrays for high-throughput analysis of DNA methylation

Pearlly S. Yan, Chuan Mu Chen, Huidong Shi, Farahnaz Rahmatpanah, Susan H. Wei, Tim Hui Ming Huang

Research output: Contribution to journalArticlepeer-review

65 Scopus citations

Abstract

Differential methylation hybridization (DMH) is a high-throughput microarray technique designed to identify changes in DNA methylation patterns commonly observed in cancer and other disease states. The DMH methodology comprises three fundamental components: the arraying of CpG island clones on glass slides, the preparation of the sample amplicons under investigation, and the hybridization of amplicon targets onto the CpG island microarray. Herein, we outline the DMH protocol and illustrate its utility and the validation approaches used in analyzing the hypermethylation profile of breast tumor and normal samples.

Original languageEnglish (US)
Pages (from-to)2430S-2434S
JournalJournal of Nutrition
Volume132
Issue number8 SUPPL.
DOIs
StatePublished - 2002

Keywords

  • CpG islands
  • DNA methylation
  • Microarray

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

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