Application of immunohistochemistry to cytology

Larry J. Fowler, Whitney A. Lachar

Research output: Contribution to journalReview articlepeer-review

59 Scopus citations


Context. - The uses of monoclonal antibodies via immunochemistry have been reported frequently within the literature using various methodologies with applications to cytology specimens. The direct application of immunocnemistry to cytology may have a variety of pitfalls that the general pathologist familiar with its application to histology may be unaware of when applying it prospectively to patient specimens. Objective. - To review common pitfalls when applying immunochemistry to cytology specimens and to suggest approaches to the more common differential dilemmas that apply to a variety of cytology specimens that could be seen in a general pathology practice. Data Sources. - The authors' own experiences of applying immunochemistry to cytopathology specimens within an academic setting along with supportive data from the literature. Conclusions. - Immunochemistry can be used to increase the predictability of a cytology diagnosis if care is taken with the cytology sample preparation methodology and there is judicious use of select monoclonal antibody panels to support a specific cytology diagnosis. Up-to-date evidence-based antibody databases should be used when selecting antibody panels.

Original languageEnglish (US)
Pages (from-to)373-383
Number of pages11
JournalArchives of Pathology and Laboratory Medicine
Issue number3
StatePublished - Mar 2008

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Medical Laboratory Technology


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