Apolipophorin-III-like protein expressed in the antenna of the red imported fire ant, Solenopsis invicta Buren (Hymenoptera: Formicidae)

Kalyani V.P. Guntur, Daniel Velasquez, Linda Chadwell, Christopher Carroll, Susan Weintraub, J. Aaron Cassill, Robert Renthal

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


Antennal proteins of the mole fire ant (Solenopsis invicta) were analyzed by two-dimensional gel electrophoresis, with the objective of identifying pheromone-binding proteins, which have not previously been found in ant antennae. The major low-molecular weight protein found in the male fire ant antenna was subjected to Edman degradation to determine the N-terminal amino acid sequence. Degenerate PCR primers based on this sequence were used to obtain a cDNA sequence corresponding to the full-length protein sequence. In-gel trypsin digestion followed by MALDI-TOF mass spectrometry and HPLC-ESI/MS/MS demonstrated that the protein gel spot contained only the protein corresponding to the cDNA sequence obtained by PCR. The sequence is similar to apolipophorin-III, an exchangeable lipid-binding protein. Fire ant apolipophorin-III is expressed in the antenna as well as the head, thorax and abdomen.

Original languageEnglish (US)
Pages (from-to)101-110
Number of pages10
JournalArchives of Insect Biochemistry and Physiology
Issue number3
StatePublished - Nov 2004


  • Apolipophorin
  • Encapsulins
  • Moss spectrometry
  • Olfoction
  • Pheromone-binding proteins

ASJC Scopus subject areas

  • Insect Science
  • Physiology
  • Biochemistry


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