Apo B insertion/deletion polymorphisms are associated with atherosclerosis in young black but not young white males

James E. Hixson, C. Alex McMahan, Henry C. McGill, Jack P. Strong

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Investigators in eight communities collected aortas, right coronary arteries, blood and liver samples, and associated information from 872 young males, aged 15-34 years, who died of external causes. Pathologists graded the arteries for atherosclerotic lesions, and a central laboratory measured lipoprotein cholesterol concentrations. Apolipoprotein (apo) B sequences were amplified in hepatic DNA samples to determine genotypes for length polymorphisms in the signal peptide of apo B. In addition to the insertion (ins) allele (27-amino acid signal peptide) and the deletion (del) allele (24 amino acids), we detected a rare allele (ins) in whites with an in-frame insertion of two Leu codons in a region that normally contains six Leu codons. The frequency for the apo B del allele was lower in blacks than in whites (p<0.0001). In blacks, homozygotes for the ins allele had the lowest levels of serum cholesterol and very low plus low density lipoprotein cholesterol (VLDL+LDL-C), homozygotes for the del allele had the highest levels, and heterozygotes had intermediate levels (p=0.0509 for cholesterol, p=0.0530 for VLDL+LDL-C), but no differences were found in whites. In blacks, homozygotes for the ins allele had the least involvement of the thoracic and the abdominal aorta with lesions, homozygotes for the del allele had the greatest involvement, and heterozygotes had intermediate involvement ( p=0.0328 for thoracic aorta, p=0.0104 for abdominal aorta), but no differences were found in whites. In blacks, apo B ins/del genotype accounted for 1.2% of the observed variation in lesions of the thoracic aorta and 1.7% of the variation in those of the abdominal aorta. The association of apo B ins/del genotypes with lesions in young blacks but not in young whites may contribute to explaining the excess of fatty streaks previously observed in young blacks.

Original languageEnglish (US)
Pages (from-to)1023-1029
Number of pages7
JournalArteriosclerosis, Thrombosis, and Vascular Biology
Volume12
Issue number9
StatePublished - 1992
Externally publishedYes

Fingerprint

Apolipoproteins B
Atherosclerosis
Alleles
Homozygote
Abdominal Aorta
Thoracic Aorta
Genotype
Heterozygote
Protein Sorting Signals
Codon
Cholesterol
Amino Acids
Liver
LDL Cholesterol
Aorta
Coronary Vessels
Arteries
Research Personnel
DNA
Serum

Keywords

  • Abdominal aorta
  • Apolipoprotein B
  • Apolipoprotein E
  • Atherosclerosis
  • Lipoprotein cholesterol
  • Polymorphisms
  • Right coronary artery
  • Serum cholesterol
  • Thoracic aorta

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Apo B insertion/deletion polymorphisms are associated with atherosclerosis in young black but not young white males. / Hixson, James E.; Alex McMahan, C.; McGill, Henry C.; Strong, Jack P.

In: Arteriosclerosis, Thrombosis, and Vascular Biology, Vol. 12, No. 9, 1992, p. 1023-1029.

Research output: Contribution to journalArticle

Hixson, James E. ; Alex McMahan, C. ; McGill, Henry C. ; Strong, Jack P. / Apo B insertion/deletion polymorphisms are associated with atherosclerosis in young black but not young white males. In: Arteriosclerosis, Thrombosis, and Vascular Biology. 1992 ; Vol. 12, No. 9. pp. 1023-1029.
@article{b74411e9ad8a4eefb04a4d407b52abc2,
title = "Apo B insertion/deletion polymorphisms are associated with atherosclerosis in young black but not young white males",
abstract = "Investigators in eight communities collected aortas, right coronary arteries, blood and liver samples, and associated information from 872 young males, aged 15-34 years, who died of external causes. Pathologists graded the arteries for atherosclerotic lesions, and a central laboratory measured lipoprotein cholesterol concentrations. Apolipoprotein (apo) B sequences were amplified in hepatic DNA samples to determine genotypes for length polymorphisms in the signal peptide of apo B. In addition to the insertion (ins) allele (27-amino acid signal peptide) and the deletion (del) allele (24 amino acids), we detected a rare allele (ins) in whites with an in-frame insertion of two Leu codons in a region that normally contains six Leu codons. The frequency for the apo B del allele was lower in blacks than in whites (p<0.0001). In blacks, homozygotes for the ins allele had the lowest levels of serum cholesterol and very low plus low density lipoprotein cholesterol (VLDL+LDL-C), homozygotes for the del allele had the highest levels, and heterozygotes had intermediate levels (p=0.0509 for cholesterol, p=0.0530 for VLDL+LDL-C), but no differences were found in whites. In blacks, homozygotes for the ins allele had the least involvement of the thoracic and the abdominal aorta with lesions, homozygotes for the del allele had the greatest involvement, and heterozygotes had intermediate involvement ( p=0.0328 for thoracic aorta, p=0.0104 for abdominal aorta), but no differences were found in whites. In blacks, apo B ins/del genotype accounted for 1.2{\%} of the observed variation in lesions of the thoracic aorta and 1.7{\%} of the variation in those of the abdominal aorta. The association of apo B ins/del genotypes with lesions in young blacks but not in young whites may contribute to explaining the excess of fatty streaks previously observed in young blacks.",
keywords = "Abdominal aorta, Apolipoprotein B, Apolipoprotein E, Atherosclerosis, Lipoprotein cholesterol, Polymorphisms, Right coronary artery, Serum cholesterol, Thoracic aorta",
author = "Hixson, {James E.} and {Alex McMahan}, C. and McGill, {Henry C.} and Strong, {Jack P.}",
year = "1992",
language = "English (US)",
volume = "12",
pages = "1023--1029",
journal = "Arteriosclerosis, Thrombosis, and Vascular Biology",
issn = "1079-5642",
publisher = "Lippincott Williams and Wilkins",
number = "9",

}

TY - JOUR

T1 - Apo B insertion/deletion polymorphisms are associated with atherosclerosis in young black but not young white males

AU - Hixson, James E.

AU - Alex McMahan, C.

AU - McGill, Henry C.

AU - Strong, Jack P.

PY - 1992

Y1 - 1992

N2 - Investigators in eight communities collected aortas, right coronary arteries, blood and liver samples, and associated information from 872 young males, aged 15-34 years, who died of external causes. Pathologists graded the arteries for atherosclerotic lesions, and a central laboratory measured lipoprotein cholesterol concentrations. Apolipoprotein (apo) B sequences were amplified in hepatic DNA samples to determine genotypes for length polymorphisms in the signal peptide of apo B. In addition to the insertion (ins) allele (27-amino acid signal peptide) and the deletion (del) allele (24 amino acids), we detected a rare allele (ins) in whites with an in-frame insertion of two Leu codons in a region that normally contains six Leu codons. The frequency for the apo B del allele was lower in blacks than in whites (p<0.0001). In blacks, homozygotes for the ins allele had the lowest levels of serum cholesterol and very low plus low density lipoprotein cholesterol (VLDL+LDL-C), homozygotes for the del allele had the highest levels, and heterozygotes had intermediate levels (p=0.0509 for cholesterol, p=0.0530 for VLDL+LDL-C), but no differences were found in whites. In blacks, homozygotes for the ins allele had the least involvement of the thoracic and the abdominal aorta with lesions, homozygotes for the del allele had the greatest involvement, and heterozygotes had intermediate involvement ( p=0.0328 for thoracic aorta, p=0.0104 for abdominal aorta), but no differences were found in whites. In blacks, apo B ins/del genotype accounted for 1.2% of the observed variation in lesions of the thoracic aorta and 1.7% of the variation in those of the abdominal aorta. The association of apo B ins/del genotypes with lesions in young blacks but not in young whites may contribute to explaining the excess of fatty streaks previously observed in young blacks.

AB - Investigators in eight communities collected aortas, right coronary arteries, blood and liver samples, and associated information from 872 young males, aged 15-34 years, who died of external causes. Pathologists graded the arteries for atherosclerotic lesions, and a central laboratory measured lipoprotein cholesterol concentrations. Apolipoprotein (apo) B sequences were amplified in hepatic DNA samples to determine genotypes for length polymorphisms in the signal peptide of apo B. In addition to the insertion (ins) allele (27-amino acid signal peptide) and the deletion (del) allele (24 amino acids), we detected a rare allele (ins) in whites with an in-frame insertion of two Leu codons in a region that normally contains six Leu codons. The frequency for the apo B del allele was lower in blacks than in whites (p<0.0001). In blacks, homozygotes for the ins allele had the lowest levels of serum cholesterol and very low plus low density lipoprotein cholesterol (VLDL+LDL-C), homozygotes for the del allele had the highest levels, and heterozygotes had intermediate levels (p=0.0509 for cholesterol, p=0.0530 for VLDL+LDL-C), but no differences were found in whites. In blacks, homozygotes for the ins allele had the least involvement of the thoracic and the abdominal aorta with lesions, homozygotes for the del allele had the greatest involvement, and heterozygotes had intermediate involvement ( p=0.0328 for thoracic aorta, p=0.0104 for abdominal aorta), but no differences were found in whites. In blacks, apo B ins/del genotype accounted for 1.2% of the observed variation in lesions of the thoracic aorta and 1.7% of the variation in those of the abdominal aorta. The association of apo B ins/del genotypes with lesions in young blacks but not in young whites may contribute to explaining the excess of fatty streaks previously observed in young blacks.

KW - Abdominal aorta

KW - Apolipoprotein B

KW - Apolipoprotein E

KW - Atherosclerosis

KW - Lipoprotein cholesterol

KW - Polymorphisms

KW - Right coronary artery

KW - Serum cholesterol

KW - Thoracic aorta

UR - http://www.scopus.com/inward/record.url?scp=0026688234&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026688234&partnerID=8YFLogxK

M3 - Article

C2 - 1525116

AN - SCOPUS:0026688234

VL - 12

SP - 1023

EP - 1029

JO - Arteriosclerosis, Thrombosis, and Vascular Biology

JF - Arteriosclerosis, Thrombosis, and Vascular Biology

SN - 1079-5642

IS - 9

ER -