TY - JOUR
T1 - Antisense Oligonucleotides from the Stage-specific Myeloid Zinc Finger Gene MZF-1 Inhibit Granulopoiesis In Vitro
AU - Bavisotto, Linda
AU - Kaushansky, Kenneth
AU - Lin, Nancy
AU - Hromas, Robert
PY - 1991/11/1
Y1 - 1991/11/1
N2 - Zinc finger proteins are transcriptional regulators of other genes, often controlling developmental cascades of gene expression. A recently cloned zinc finger gene, MZF-1, was found to be preferentially expressed in myeloid cells. Using complementary radiolabeled MZF-1 tLNA hybridized to human bone marrow smears in situ, it was discovered that the expression of MZF-1 is essentially limited to the myelocyte and metamyelocyte stages of granulopoiesis. Antisense but not sense oligonucleotides from MZF-1 significantly inhibited granulocyte colony-stimulating factor-driven granulocyte colony formation in vitro.
AB - Zinc finger proteins are transcriptional regulators of other genes, often controlling developmental cascades of gene expression. A recently cloned zinc finger gene, MZF-1, was found to be preferentially expressed in myeloid cells. Using complementary radiolabeled MZF-1 tLNA hybridized to human bone marrow smears in situ, it was discovered that the expression of MZF-1 is essentially limited to the myelocyte and metamyelocyte stages of granulopoiesis. Antisense but not sense oligonucleotides from MZF-1 significantly inhibited granulocyte colony-stimulating factor-driven granulocyte colony formation in vitro.
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U2 - 10.1084/jem.174.5.1097
DO - 10.1084/jem.174.5.1097
M3 - Article
C2 - 1719120
AN - SCOPUS:0025953387
VL - 174
SP - 1097
EP - 1101
JO - Journal of Experimental Medicine
JF - Journal of Experimental Medicine
SN - 0022-1007
IS - 5
ER -